RPL22 Antibody (OAEB00219)

Catalog No: OAEB00219 (Formerly GWB-6DD8DC)

Size:100UG

OAEB00219 (3.8ug/ml) staining of paraffin embedded Human Cortex Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Info

• Predicted Species Reactivity: Human|Mouse|Rat
• Product Format: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Clonality: Polyclonal
• Host: Goat
• Application: ELISA|IHC
• Reconstitution and Storage: -20°C
• Immunogen: Ribosomal protein L22
• Purification: Ammonium Sulfate Precipitated; Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Cow: 100%; Dog: 100%; Guinea Pig: 84%; Horse: 84%; Human: 100%; Mouse: 100%; Rat: 100%; Zebrafish: 92%
• Peptide Sequence: C-SKESYELRYFQINQ
• Concentration: 0.5 mg/ml
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Application Info:
  Peptide ELISA: antibody detection limit dilution 1:16000.
  Western Blot: Preliminary experiments gave bands at approx 17-18kDa and 25kDa in Human Liver lysates after 0.5ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 14.8kDa according to NP_000974.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands).
  IHC: In paraffin embedded Human Brain (Cortex) shows cytoplasm staining in neuronal bodies. Recommended concentration, 3-6ug/ml

Target Info

• Reference: * Anderson SJ, Lauritsen JP, Hartman MG, Foushee AM, Lefebvre JM, Shinton SA, Gerhardt B, Hardy RR, Oravecz T, Wiest DL.; Ablation of Ribosomal Protein L22 Selectively Impairs alphabeta T Development by Activation of a p53-Dependent Checkpoint.; Immunity. 2007 Jun;26(6):759-72. Epub 2007 Jun 7.; Pubmed 17555992
• Gene Symbol: Rpl22|RPL22
• Gene Full Name: ribosomal protein L22
• Alias Symbols: 2700038K18Rik, 60S ribosomal protein L22, EAP, EBER-associated protein, Epstein-Barr virus small RNA-associated protein, Epstein-Barr-encoded RNA-associated protein, HBP15, HBP15/L22, heparin-binding protein 15, heparin-binding protein HBp15, L22, large ribosomal subunit protein eL22.
• NCBI Gene Id: 19934|6146|81768
• Protein Name: 60S ribosomal protein L22 [Homo sapiens]
• Uniprot ID: P35268
• Protein Accession #: https://www.ncbi.nlm.nih.gov/protein/NP_000974.1
• Molecular Weight: 14.8