RPIA ELISA Kit (Human) (OKEH02290)

Catalog No: OKEH02290

Size:96T

Human RPIA ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: ELISA-Comp
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology RPIA ELISA Kit (Human) (OKEH02290) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Human RPIA antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated RPIA and incubated. The RPIA found in the sample or standards competes with the biotinylated RPIA for limited binding sites on the immobilized anti-Human RPIA antibody. Excess unbound biotinylated RPIA and sample or standard RPIA is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated RPIA captured in the well and inversely proportional to the amount of RPIA which was contained in the sample or standard.
• ELISA Kit Range: 1.56-100ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 90%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: < 5.4% (n = 20)
  Mean Inter-assay CV%: < 10.8% (n = 20)
• ELISA Kit Component:
  • Anti-RPIA Microplate: 96 Wells (12 x 8 Well strips)
  • RPIA Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • 100X RPIA Biotin Complex: 1 x 60 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Biotin Complex Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 0.95 ng/mL
• Specificity: Natural and recombinant Human Ribose-5-phosphate isomerase
• Assay Info: Assay Methodology: Quantitative Competitive ELISA

Target Info

• Gene Symbol: RPIA
• Gene Full Name: ribose 5-phosphate isomerase A
• Alias Symbols: phosphoriboisomerase;ribose 5-phosphate epimerase;ribose-5-phosphate isomerase;RPI;RPIAD.
• NCBI Gene Id: 22934
• Protein Name: P49247
• Description of Target: Ribose-5-phosphate isomerase
• Uniprot ID: https://www.uniprot.org/uniprot/P49247
• Protein Accession #: NP_653164.2
• Nucleotide Accession #: NM_144563.2
• Protein Size (# AA): 311
• Molecular Weight: 33 kDa