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Catalog No: OKEH02018
Size:96T
Price: $600.00
SKU
OKEH02018
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology RPE65 ELISA Kit (Human) (OKEH02018) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for RPE65 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for RPE65 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample RPE65 captured in well.
ELISA Kit Range78-5000pg/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 81%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 4.7% (n = 20)
Mean Inter-assay CV%: < 6.9% (n = 20)
ELISA Kit Component
ComponentAmount
RPE65 Microplate96 Wells (12 x 8 Well strips)
RPE65 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated RPE65 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity32 pg/mL
SpecificityNatural and recombinant Human Retinoid isomerohydrolase
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolRPE65
Gene Full Nameretinoid isomerohydrolase RPE65
Alias Symbolsall-trans-retinyl-palmitate hydrolase;BCO family, member 3;BCO3;LCA2;lutein isomerase;meso-zeaxanthin isomerase;mRPE65;p63;RBP-binding membrane protein;rd12;retinal pigment epithelium specific protein 65;retinal pigment epithelium-specific 65 kDa protein;retinal pigment epithelium-specific protein 65kDa;retinitis pigmentosa 20 (autosomal recessive);retinoid isomerohydrolase;retinol isomerase;RP20;RPE65, retinoid isomerohydrolase;sRPE65.
NCBI Gene Id6121
Protein NameQ16518
Description of TargetRetinoid isomerohydrolase
Uniprot IDhttps://www.uniprot.org/uniprot/Q16518
Protein Accession #NP_000320.1
Nucleotide Accession #NM_000329.2
Protein Size (# AA)533
Molecular Weight60 kDa
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