RPA1 Antibody (OAAB22061)

Catalog No: OAAB22061

Size:100ul

Western blot detection of RPA70 in Hela, A431, MCF7, COS7, HT1080 and K562 cell lysates using RPA70 Antibody (OAAB22061) (1:1000 diluted). Predicted band size: 70KDa. Observed band size: 70KDa.

Immunocytochemistry staining of HeLa cells fixed in 1% Paraformaldehyde and using RPA70 Antibody (OAAB22061) (dilution 1:100).

Immunoprecipitation analysis of Hela cell lysates using RPA70 Antibody (OAAB22061).

Product Info

• Predicted Species Reactivity: Human, Mouse
• Product Format: Liquid
• Clonality: Monoclonal
• Host: Rabbit
• Application: WB, ICC, IP
• Additional Information: Function: As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates, that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage (PubMed:9430682). In the cellular response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation. Through recruitment of ATRIP activates the ATR kinase a master regulator of the DNA damage response (PubMed:24332808). It is required for the recruitment of the DNA double-strand break repair factors RAD51 and RAD52 to chromatin in response to DNA damage (PubMed:17765923). Also recruits to sites of DNA damage proteins like XPA and XPG that are involved in nucleotide excision repair and is required for this mechanism of DNA repair (PubMed:7697716). Plays also a role in base excision repair (BER) probably through interaction with UNG (PubMed:9765279). Through RFWD3 may activate CHEK1 and play a role in replication checkpoint control. Also recruits SMARCAL1/HARP, which is involved in replication fork restart, to sites of DNA damage. May also play a role in telomere maintenance (PubMed:17959650). As part of the alternative replication protein A complex, aRPA, binds single-stranded DNA and probably plays a role in DNA repair. Compared to the RPA2- containing, canonical RPA complex, may not support chromosomal DNA replication and cell cycle progression through S-phase. The aRPA may not promote efficient priming by DNA polymerase alpha but could support DNA synthesis by polymerase delta in presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange (PubMed:19996105).
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Predicted Homology Based on Immunogen Sequence: Human, Mouse
• Preservative: 0.02% Sodium Azide and 50% glycerol
• Formulation: Purified mouse monoclonal antibody in PBS (pH 7.4) containing with 0.02% sodium azide and 50% glycerol.
• Application Info: WB: 1:1000
  ICC: 1:100
  IP: 1:500
• Reference: Erdile L.F.,et al.J. Biol. Chem. 266:12090-12098(1991).
  Erdile L.F.,et al.J. Biol. Chem. 268:2268-2268(1993).
  Ota T.,et al.Nat. Genet. 36:40-45(2004).
  Totoki Y.,et al.Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases.
  Mural R.J.,et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
  

Target Info

• Gene Symbol: RPA1
• Gene Full Name: replication protein A1
• Alias Symbols: HSSB, RF-A, RP-A, REPA1, RPA70, MST075
• NCBI Gene Id: 6117
• Uniprot ID: P27694
• Protein Accession #: NP_002936.1
• Nucleotide Accession #: NM_002945.3
• Molecular Weight: 68 kDa