Catalog No: OKEH02106
Size:96T
Price: $375.00
SKU
OKEH02106
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RORC ELISA Kit (Human) : 96 Wells (OKEH02106)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Competitive
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology RORC ELISA Kit (Human) (OKEH02106) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Human RORC antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated RORC and incubated. The RORC found in the sample or standards competes with the biotinylated RORC for limited binding sites on the immobilized anti-Human RORC antibody. Excess unbound biotinylated RORC and sample or standard RORC is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated RORC captured in the well and inversely proportional to the amount of RORC which was contained in the sample or standard.
ELISA Kit Range0.156-10ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 98%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 4.7% (n = 20)
Mean Inter-assay CV%: < 6.9% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-RORC Microplate96 Wells (12 x 8 Well strips)
RORC Lyophilized Standard2
Sample Diluent1 x 20 mL
100X PDK1-Biotin Complex1 x 60 uL
100X Avidin-HRP Conjugate1 x 120 uL
Biotin Complex Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity0.078 ng/mL
SpecificityNatural and recombinant Human Nuclear receptor ROR-gamma
Assay InfoAssay Methodology: Quantitative Competitive ELISA
Gene SymbolRORC
Gene Full NameRAR related orphan receptor C
Alias SymbolsIMD42;NR1F3;nuclear receptor ROR-gamma;nuclear receptor RZR-gamma;nuclear receptor subfamily 1 group F member 3;RAR-related orphan nuclear receptor variant 2;RAR-related orphan receptor C;retinoic acid-binding receptor gamma;retinoid-related orphan receptor gamma;Retinoid-related orphan receptor-gamma;RORG;RZRG;RZR-GAMMA;TOR.
NCBI Gene Id6097
Protein NameP51449
Description of TargetNuclear receptor ROR-gamma
Uniprot IDhttps://www.uniprot.org/uniprot/P51449
Protein Accession #NP_001001523.1
Nucleotide Accession #NM_001001523.1
Protein Size (# AA)518
Molecular Weight58 kDa
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