ROR2 Antibody - N-terminal region (OAAB17187)

Catalog No: OAAB17187

Size:400ul

Western blot analysis of ROR2 (arrow) using rabbit polyclonal ROR2 Antibody (N-term) (Cat.#OAAB17187). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ROR2 gene (Lane 2) (Origene Technologies).

Formalin-fixed and paraffin-embedded human kidney carcionma reacted with ROR2 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Flow cytometric analysis of NCI-H292 cells using ROR2 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Clonality: Polyclonal
• Isotype: Ig
• Host: Rabbit
• Application: WB, IHC, FC
• Additional Information: Function: Tyrosine-protein kinase receptor which may be involved in the early formation of the chondrocytes. It seems to be required for cartilage and growth plate development Phosphorylates YWHAB, leading to induction of osteogenesis and bone formation
  Cellular Location: Cell membrane; Single-pass type I membrane protein (By similarity)
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This ROR2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 19-50 amino acids from the N-terminal region of human ROR2.
• Predicted Homology Based on Immunogen Sequence: Human
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Sample Type Confirmation:

  ROR2 is strongly supported by BioGPS gene expression data to be expressed in HEK293

• Intended Use: ROR2 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
• Application Info: WB: 1:1000
  IHC-P: 1:10~50
  FC: 1:10~50

Target Info

• Gene Symbol: ROR2
• Alias Symbols: BDB, BDB1, NTRKR2
• NCBI Gene Id: 4920
• Protein Name: Tyrosine-protein kinase transmembrane receptor ROR2
• Description of Target: Purified Rabbit Polyclonal Antibody (Pab)
• Uniprot ID: Q01974
• Protein Accession #: NP_004551.2
• Molecular Weight: 104757 Da