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Datasheets/Manuals | Printable datasheet for OABB00251 |
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Tested Species Reactivity | Human, Rat |
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Predicted Species Reactivity | Mouse |
Product Format | Lyophilized |
Clonality | Polyclonal |
Clone | Polyclonal |
Isotype | Rabbit IgG |
Application | WB, IHC-P |
Additional Information | Notes: Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. |
:: | Background: Rho-associated kinase(ROCK), including the ROCK-I and ROCK-II isoforms, is a protein kinase involved in signaling from Rho to actin cytoskeleton. Serine/threonine kinase ROCK II/Rho kinase, which is an isozyme of ROCK I, is one of the targets for the small GTPase Rho. ROCK II regulates the formation of actin stress fibers and focal adhesions, cytokinesis, smooth muscle contraction, and the activation of c-fos serum response element. Sequencing analysis has shown that human ROCK II contains 1388 amino acid residues with a calculated molecular mass of approximately 161 kDa. Fluorescence in situ hybridization analysis showed that the human ROCK II gene is located on chromosome 2p24. Thumkeo et al.(2003) concluded that ROCK-II is essential in inhibiting blood coagulation and maintaining blood flow in the endothelium-free labyrinth layer and that loss of ROCK-II leads to thrombus formation, placental dysfunction, intrauterine growth retardation, and fetal death. |
:: | Background: Rho-associated kinase (ROCK), including the ROCK-I and ROCK-II isoforms, is a protein kinase involved in signaling from Rho to actin cytoskeleton. Serine/threonine kinase ROCK II/Rho kinase, which is an isozyme of ROCK I, is one of the targets for the small GTPase Rho. ROCK II regulates the formation of actin stress fibers and focal adhesions, cytokinesis, smooth muscle contraction, and the activation of c-fos serum response element. Sequencing analysis has shown that human ROCK II contains 1388 amino acid residues with a calculated molecular mass of approximately 161 kDa. Fluorescence in situ hybridization analysis showed that the human ROCK II gene is located on chromosome 2p241. Thumkeo et al. (2003) concluded that ROCK-II is essential in inhibiting blood coagulation and maintaining blood flow in the endothelium-free labyrinth layer and that loss of ROCK-II leads to thrombus formation, placental dysfunction, intrauterine growth retardation, and fetal death.2 |
Reconstitution and Storage | Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml.. At -20C for one year from date of receipt. After reconstitution, at 4C for one month. It can also be aliquotted and stored frozen at -20C for six months. Avoid repeated freezing and thawing. |
Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human ROCK2(35-51aa RDPRSPINVESLLDGLN), identical to the related rat and mouse sequences. |
Purification | Immunogen affinity purified. |
Predicted Homology Based on Immunogen Sequence | Human; Mouse; Rat |
Specificity | No cross reactivity with other proteins. |
Application Info | Western blot: 0.1-0.5 ug/ml: Human, Rat: Mouse Immunohistochemistry (Paraffin-embedded Section): 0.5-1 ug/ml: Human: Mouse, Rat: By Heat |
Reference | 1) Takahashi, N.; Tuiki, H.; Saya, H.; Kaibuchi, K. : Localization of the gene coding for ROCK II/Rhokinase on human chromosome 2p24. Genomics 55: 235-237, 1999. 2) Thumkeo, D.; Keel, J.; Ishizaki, T.; Hirose, M.; Nonomura, K.; Oshima, H.; Oshima, M.; Taketo, M. M.; Narumiya, S. : Targeted disruption of the mouse Rho-associated kinase 2 gene results in intrauterine growth retardation and fetal death. Molec. Cell. Biol. 23: 5043-5055, 2003. |
Storage Buffer | Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. |
Description | Rabbit IgG polyclonal antibody for Rho-associated protein kinase 2(ROCK2) detection. Tested with WB, IHC-P in Human;Mouse;Rat. |
Gene Symbol | ROCK2 |
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Gene Full Name | Rho-associated, coiled-coil containing protein kinase 2 |
Alias Symbols | coiled-coil-containing protein kinase 2, KIAA0619, p164 ROCK 2, p164 ROCK-2, Rho associated coiled coil containing protein kinase 2, Rho associated protein kinase 2, Rho associated, coiled coil containing protein kinase II, Rho kinase 2, Rho-associated, Rho-associated protein kinase 2, ROCK 2, Rock II, Rock2, ROCK2_HUMAN, Rock2m, ROK alpha, ROKalpha |
Protein Name | Rho-associated protein kinase 2 |
Uniprot ID | O75116 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "ROCK2 Polyclonal Antibody (OABB00251)"?
The tested species reactivity for this item is "Human, Rat". This antibody is predicted to have homology to "Mouse".
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How long will it take to receive "ROCK2 Polyclonal Antibody (OABB00251)"?
This item is available "Domestic: within 1-2 week delivery | International: within 1-2 week delivery".
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What buffer format is "ROCK2 Polyclonal Antibody (OABB00251)" provided in?
This item is provided in "Lyophilized".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "ROCK2 Polyclonal Antibody (OABB00251)"?
This target may also be called "coiled-coil-containing protein kinase 2, KIAA0619, p164 ROCK 2, p164 ROCK-2, Rho associated coiled coil containing protein kinase 2, Rho associated protein kinase 2, Rho associated, coiled coil containing protein kinase II, Rho kinase 2, Rho-associated, Rho-associated protein kinase 2, ROCK 2, Rock II, Rock2, ROCK2_HUMAN, Rock2m, ROK alpha, ROKalpha" in publications.
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What is the shipping cost for "ROCK2 Polyclonal Antibody (OABB00251)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "ROCK2 Polyclonal Antibody (OABB00251)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "ROCK2 Polyclonal Antibody (OABB00251)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "ROCK2 Polyclonal Antibody (OABB00251)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "ROCK2"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "ROCK2"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "ROCK2"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "ROCK2"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "ROCK2"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "ROCK2"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.