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Catalog No: OKEH04726
Size:96T
Price: $525.00
SKU
OKEH04726
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
COA DatasheetPrintable COA datasheet for OKEH04726
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~3 Hours
ELISA Kit PrincipleAviva Systems Biology ROCK2 ELISA Kit (Human) (OKEH04726) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ROCK2 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ROCK2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ROCK2 captured in well.
ELISA Kit Range1.56-100ng/mL
ELISA Kit ReproducibilityMean Intra-Assay CV: < 10%
Mean Inter-Assay CV: < 12%
ELISA Kit Component
ComponentAmount
ROCK2 Microplate96 Wells (12 x 8 Well Strips)
ROCK2 Standard2
Sample Diluent1 x 20 mL
ROCK2 Biotinylated Detector Antibody1 x 120 uL
Detector Antibody Diluent1 x 12 mL
HRP Avidin Conjugate1 x 120 uL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.
Sensitivity0.76 ng/mL
SpecificityNatural and recombinant Human Rho-associated protein kinase 2
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolROCK2
Gene Full NameRho associated coiled-coil containing protein kinase 2
Alias Symbolsp164 ROCK-2;Rho kinase 2;rho-associated protein kinase 2;Rho-associated, coiled-coil-containing protein kinase 2;rho-associated, coiled-coil-containing protein kinase II;ROCK-II.
NCBI Gene Id9475
Protein NameO75116
Description of TargetRho-associated protein kinase 2
Uniprot IDhttps://www.uniprot.org/uniprot/O75116
Protein Accession #NP_001308572.1
Nucleotide Accession #NM_001321643.1
Protein Size (# AA)1388
Molecular Weight161 kDa
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