ROCK1 Antibody (OAGA00998)

Catalog No: OAGA00998

Size:100 ul

Sample (30 ug of whole cell lysate)
A: A431
B: H1299
C: Molt-4
5% SDS PAGE
OAGA00998 diluted at 1:1000

ROCK1 antibody [N1N2], N-term detects ROCK1 protein by Western blot analysis.
A. 50 ug rat brain lysate/extract
5 % SDS-PAGE
ROCK1 antibody [N1N2], N-term (OAGA00998) dilution: 1:500

ROCK1 antibody [N1N2], N-term detects ROCK1 protein by western blot analysis.
A. 30 ug NIH-3T3 whole cell extract
B. 30 ug C2Cl2 whole cell extract
5 % SDS-PAGE ROCK1 antibody [N1N2], N-term (OAGA00998) dilution: 1:1000

ROCK1 antibody [N1N2], N-term detects ROCK1 protein at cytoplasm on human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
ROCK1 antibody [N1N2], N-term (OAGA00998) diluted at 1:500.

ROCK1 antibody [N1N2], N-term detects ROCK1 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ROCK1 protein stained by ROCK1 antibody [N1N2], N-term (OAGA00998) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody diluted at 1:500. Blue: Hoechst 33342 staining.

ROCK1 antibody immunoprecipitates ROCK1 protein in IP experiments. IP Sample: A431 whole cell lysate/extract
A : Control with 3 ug of pre-immune rabbit IgG
B : Immunoprecipitation of ROCK1 by 3 ug of ROCK1 antibody (OAGA00998) 5% SDS-PAGE The immunoprecipitated ROCK1 protein was detected by ROCK1 antibody (OAGA00998) diluted at 1:1000. EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: IP, ICC, IF, IHC, WB
  Positive Controls: A431, H1299, Molt-4, rat brain, NIH-3T3 , C2C12
  Cellular Localization: Cytoplasm, Centrosome, centriole (By similarity), Golgi apparatus membrane; Peripheral membrane protein
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Recombinant protein encompassing a sequence within the N-terminus region of human ROCK1. The exact sequence is proprietary.
• Purification: Purified by antigen-affinity chromatography.
• Concentration: 0.68 mg/ml
• Application Info: Suggested starting dilutions are as follows:
  WB: 1:500-1:3000,
  IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)],
  ICC/IF: 1:100-1:1000.
  IP: 1:100-1:500.
  Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
• Storage Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Target Info

• Gene Symbol: ROCK1
• Gene Full Name: Rho-associated protein kinase 1
• Alias Symbols: ROCK-I, P160ROCK
• NCBI Gene Id: 6093
• Protein Name: Rho-associated protein kinase 1
• Description of Target: This gene encodes a protein serine/threonine kinase that is activated when bound to the GTP-bound form of Rho. The small GTPase Rho regulates formation of focal adhesions and stress fibers of fibroblasts, as well as adhesion and aggregation of platelets and lymphocytes by shuttling between the inactive GDP-bound form and the active GTP-bound form. Rho is also essential in cytokinesis and plays a role in transcriptional activation by serum response factor. This protein, a downstream effector of Rho, phosphorylates and activates LIM kinase, which in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. [provided by RefSeq]
• Uniprot ID: Q13464
• Protein Accession #: NP_005397.1
• Molecular Weight: 158 kDa