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ROBO3 Antibody (OAEB00015)

100 ug
$349.00
In Stock
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Predicted Species Reactivity:
Dog, Human
Product Format:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Clonality:
Polyclonal
Host:
Goat
Application:
ELISA
:::
Reactivity Info: Expected: Human, Dog
Reconstitution and Storage:
Aliquot and store at -20C. Minimize freezing and thawing.
Gene Symbol:
ROBO3
NCBI Gene Id:
64221
Protein Name:
Roundabout homolog 3
Swissprot Id:
Q96MS0
Protein Accession #:
NP_071765.2
Alias Symbols:
Goat Anti-ROBO3 / RIG1 (internal) Antibody, roundabout, axon guidance receptor, homolog 3, retinoblastoma inhibiting gene 1, RIG1, RBIG1, HGPPS, FLJ21044, HGNC:13433, roundabout, axon guidance receptor, homolog 3 (Drosophila), ROBO3
Replacement Item:
This antibody may replace item sc-44498 from Santa Cruz Biotechnology.
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express ROBO3.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express ROBO3.
Immunogen:
The immunogen for anti-ROBO3 antibody: peptide with sequence C-RKDGARLKEEEGR, from the internal region of the protein sequence according to NP_071765.2.
Predicted Homology Based on Immunogen Sequence:
Dog; Human
Concentration:
100ug specific antibody in 200ul
Datasheets/Manuals:
Printable datasheet for anti-ROBO3 antibody - OAEB00015
Purity:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Application Info:
Peptide ELISA: Antibody detection limit dilution 1:64000.
Western Blot: Preliminary experiments gave an approx 25kDa band in Human Brain lysates after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 148kDa according to NP_071765.2. The 25kDa band was successfully blocked by incubation with the immunizing peptide.

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