RNH1 ELISA Kit (Pig) (OKEH08026)

Catalog No: OKEH08026

Size:96T

Pig RNH1 ELISA Standard Curve

Product Info

• Predicted Species Reactivity: Porcine, Sus scrofa
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Principle: Aviva Systems Biology RNH1 ELISA Kit (Pig) (OKEH08026) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for RNH1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for RNH1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample RNH1 captured in well.
• ELISA Kit Range: 0.78-50ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 105% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=7.5% (n = 20)
  Mean Inter-assay CV%: <=8.3% (n = 20)
• ELISA Kit Component:
  • RNH1 Microplate: 96 Wells (12 x 8 Well strips)
  • RNH1 Lyophilized Standard: 2
  • 100X Biotinylated RNH1 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Function: Ribonuclease inhibitor which inhibits RNASE1, RNASE2 and ANG. May play a role in redox homeostasis.
  Subcellular Location: Cytoplasm
  Unigene: Ssc.6413
  SMR: P10775
  KEGG: ssc:445517
  Pfam: PF13516
• Reconstitution and Storage: Store as indicated in product manual.
• Sensitivity: 0.24ng/mL
• Specificity: Natural and recombinant Pig Ribonuclease inhibitor
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: RNH1
• Alias Symbols: Ribonuclease/angiogenin inhibitor 1.
• Protein Name: P10775
• Description of Target: Ribonuclease inhibitor
• Uniprot ID: https://www.uniprot.org/uniprot/P10775
• Protein Accession #: XP_013847653.1
• Nucleotide Accession #: XM_013992199.1