RNF5 Antibody - middle region (OAAB08708)

Catalog No: OAAB08708

Size:400ul

Western blot analysis of RNF5 (arrow) using rabbit polyclonal RNF5 Antibody (Center) (Cat. #OAAB08708). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the RNF5 gene.

RNF5 is supported by BioGPS gene expression data to be expressed in HEK293

RNF5 Antibody (Center) (Cat. #OAAB08708)immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of RNF5 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat
• Product Format: Liquid. PBS with 0.09% (W/V) sodium azide.
• Clonality: Polyclonal
• Isotype: Ig
• Host: Rabbit
• Application: WB, IHC-P
  Function: Has E2-dependent E3 ubiquitin-protein ligase activity. May function together with E2 ubiquitin-conjugating enzymes UBE2D1/UBCH5A and UBE2D2/UBC4. Mediates ubiquitination of PXN/paxillin and Salmonella type III secreted protein sopA. May be involved in regulation of cell motility and localization of PXN/paxillin. Mediates the 'Lys-63'-linked polyubiquitination of JKAMP thereby regulating JKAMP function by decreasing its association with components of the proteasome and ERAD; the ubiquitination appears to involve E2 ubiquitin-conjugating enzyme UBE2N. Mediates the 'Lys-48'-linked polyubiquitination of TMEM173 at 'Lys-150' leading to its proteasomal degradation; the ubiquitination occurrs in mitochondria after viral transfection and regulates antiviral response.
  Cellular Location: Membrane; Multi-pass membrane protein. Mitochondrion membrane. Endoplasmic reticulum membrane Note=Predominantly located in the plasma membrane, with some localization occurring within cytoplasmic organelles
  Tissue Location: Widely expressed.
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This RNF5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 65-94 amino acids of human RNF5.
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Predicted Homology Based on Immunogen Sequence: Human; Mouse; Rat
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Sample Type Confirmation:

  RNF5 is supported by BioGPS gene expression data to be expressed in HEK293

• Application Info: WB: 1:1000
  IHC-P: 1:10~50

Target Info

• Gene Symbol: RNF5
• Alias Symbols: RMA1, RING5
• NCBI Gene Id: 6048
• Protein Name: E3 ubiquitin-protein ligase RNF5
• Description of Target: The protein encoded by this gene contains a RING finger, which is a motif known to be involved in protein-protein interactions. This protein is a membrane-bound ubiquitin ligase. It can regulate cell motility by targeting paxillin ubiquitination and altering the distribution and localization of paxillin in cytoplasm and cell focal adhesions.
• Uniprot ID: Q99942
• Protein Accession #: NP_008844.1
• Molecular Weight: 19881 Da