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Datasheets/Manuals | Printable datasheet for anti-RNF31 (ARP43241_P050-HRP) antibody |
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Predicted Species Reactivity | Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit |
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Product Format | Liquid. Purified antibody is supplied in high phosphate PBS, 100 mm phosphate, 150 mM NaCl, pH 7.6. |
Clonality | Polyclonal |
Host | Rabbit |
Conjugation | HRP: Horseradish Peroxidase |
Application | WB |
Reconstitution and Storage | All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil). Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding. |
Immunogen | The immunogen is a synthetic peptide directed towards the middle region of human RNF31 |
Purification | Affinity Purified |
Predicted Homology Based on Immunogen Sequence | Cow: 93%; Dog: 93%; Guinea Pig: 100%; Horse: 86%; Human: 100%; Mouse: 92%; Rabbit: 100%; Rat: 100% |
Peptide Sequence | Synthetic peptide located within the following region: SLINAHSLDPATLYEVEELETATERYLHVRPQPLAGEDPPAYQARLLQKL |
Concentration | 0.5 mg/ml |
Blocking Peptide | For anti-RNF31 (ARP43241_P050-HRP) antibody is Catalog # AAP43241 (Previous Catalog # AAPP26473) |
Reference | Kirisako,T., (2006) EMBO J. 25 (20), 4877-4887 |
Publications | Queisser, M. A. et al. HOIL-1L Functions as the PKCζ Ubiquitin Ligase to Promote Lung Tumor Growth. (2014). at /www.atsjournals.org/doi/full/10.1164/rccm.201403-0463OC#.VC2t1fldWVN> WB, Human, Rabbit, Rat, Guinea pig, Bovine, Dog, Mouse, Horse 25118570 Sieber, S. et al. Sharpin contributes to TNF-alpha dependent NFκB activation and anti-apoptotic signalling in hepatocytes. PLoS One 7, e29993 (2012). WB, IP, Human, Rabbit, Rat, Guinea pig, Bovine, Dog, Mouse, Horse 22253853 Smit, J. J. et al. The E3 ligase HOIP specifies linear ubiquitin chain assembly through its RING-IBR-RING domain and the unique LDD extension. EMBO J. 31, 3833-44 (2012). WB, IP, Human, Rabbit, Rat, Guinea pig, Bovine, Dog, Mouse, Horse 22863777 Blackwell, K. et al. Two coordinated mechanisms underlie tumor necrosis factor alpha-induced immediate and delayed IκB kinase activation. Mol. Cell. Biol. 33, 1901-15 (2013). WB, IP, Human, Rabbit, Rat, Guinea pig, Bovine, Dog, Mouse, Horse 23459942 Liu, S. et al. MAVS recruits multiple ubiquitin E3 ligases to activate antiviral signaling cascades. Elife 2, e00785 (2013). WB, IP, Human, Rabbit, Rat, Guinea pig, Bovine, Dog, Mouse, Horse 23951545 |
Gene Symbol | RNF31 |
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Gene Full Name | Ring finger protein 31 |
Alias Symbols | HOIP, Paul, ZIBRA |
NCBI Gene Id | 55072 |
Protein Name | E3 ubiquitin-protein ligase RNF31 |
Description of Target | PWP2 contains a RING finger, a motif present in a variety of functionally distinct proteins and known to be involved in protein-DNA and protein-protein interactions.The protein encoded by this gene contains a RING finger, a motif present in a variety of functionally distinct proteins and known to be involved in protein-DNA and protein-protein interactions. |
Uniprot ID | Q96EP0 |
Protein Accession # | NP_060469 |
Nucleotide Accession # | NM_017999 |
Protein Size (# AA) | 1072 |
Molecular Weight | 120kDa |
Protein Interactions | CCDC24; VASN; KRTAP9-2; TSSC4; UBC; SNRPC; PRKCA; AES; PITX1; KIF1A; SHARPIN; RBCK1; RNF31; MALT1; VCP; OTULIN; IRAK1; ESR1; Ikbkg; CCDC14; WRNIP1; AKAP8L; TRAF2; TNF; NDUFA8; BIRC2; NOD2; BIRC3; HCVgp1; SOCS3; SMAD3; NOS3; UBE2D3; UBE2D1; TNFAIP3; GFI1B; |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "RNF31 Antibody - middle region : HRP (ARP43241_P050-HRP)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit".
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How long will it take to receive "RNF31 Antibody - middle region : HRP (ARP43241_P050-HRP)"?
This item is available "Domestic: within 1-2 days delivery | International: 1-2 days".
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What buffer format is "RNF31 Antibody - middle region : HRP (ARP43241_P050-HRP)" provided in?
This item is provided in "Liquid. Purified antibody is supplied in high phosphate PBS, 100 mm phosphate, 150 mM NaCl, pH 7.6.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "RNF31 Antibody - middle region : HRP (ARP43241_P050-HRP)"?
This target may also be called "HOIP, Paul, ZIBRA" in publications.
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What is the shipping cost for "RNF31 Antibody - middle region : HRP (ARP43241_P050-HRP)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "RNF31 Antibody - middle region : HRP (ARP43241_P050-HRP)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "RNF31 Antibody - middle region : HRP (ARP43241_P050-HRP)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "120kDa".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "RNF31 Antibody - middle region : HRP (ARP43241_P050-HRP)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "RNF31"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "RNF31"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "RNF31"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "RNF31"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "RNF31"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "RNF31"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.