RNASE1 Polyclonal Antibody (OABB00461)

Catalog No: OABB00461 (Formerly GWB-BBP384)

Size:100UG

Lane1: MCF-7 Cell Lysate
Lane2: U87 Cell Lysate
Lane3: Hela Cell Lysate
Lane4: MM231 Cell Lysate
Lane5: Rabbit IgG (55KD)
Lane6: Jurkat Cell Lysate
Lane7: SMMC Cell Lysate
Lane8: Colo320 Cell Lyate
Lane9: SW620 Cell Lysate
Lane10: CEM Cell Lysate

Product Info

• Tested Species Reactivity: Human
• Predicted Species Reactivity: Human
• Product Format: Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
• Clonality: Polyclonal
• Clone: Polyclonal
• Isotype: Rabbit IgG
• Application: Western blot
• Additional Information: Notes: Tested Species: In-house tested species with positive results.
  Predicted Species: Species predicted to be fit for the product based on sequence similarities.
  Other applications have not been tested.
  Optimal dilutions should be determined by end users.
  
  Background: RIBONUCLEASE A FAMILY, 1(Ribonuclease pancreatic) is an enzyme that in humans is encoded by the RNASE1 gene. By genomic sequence analysis, RNASE1 gene is mapped the to chromosome 14q11.2, where it is linked to 7 other RNase A superfamily genes. Monomeric human pancreatic RNase, devoid of any biological activity other than its RNA degrading ability, was engineered into a dimeric protein with a cytotoxic action on mouse and human tumor cells, but lacking any appreciable toxicity on mouse and human normal cells. This dimeric variant of human pancreas RNase selectively sensitizes to apoptotic death cells derived from a human thyroid tumor. Because of its selectivity for tumor cells, and because of its human origin, this protein represents a potentially very attractive, novel tool for anticancer therapy.
  Background: RIBONUCLEASE A FAMILY, 1 (Ribonuclease pancreatic) is an enzyme that in humans is encoded by the RNASE1 gene. By genomic sequence analysis, RNASE1 gene is mapped the to chromosome 14q11.2, where it is linked to 7 other RNase A superfamily genes. Monomeric human pancreatic RNase, devoid of any biological activity other than its RNA degrading ability, was engineered into a dimeric protein with a cytotoxic action on mouse and human tumor cells, but lacking any appreciable toxicity on mouse and human normal cells. This dimeric variant of human pancreas RNase selectively sensitizes to apoptotic death cells derived from a human thyroid tumor. Because of its selectivity for tumor cells, and because of its human origin, this protein represents a potentially very attractive, novel tool for anticancer therapy.
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Immunogen: Polypeptide
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Human
• Concentration: 500 ug/ml
• Specificity: No cross reactivity with other proteins.
• Application Info: Western blot: 0.1-0.5 ug/ml: Human
• Reference: 1. Zhang, J., Dyer, K. D., Rosenberg, H. F. RNase 8, a novel RNase A superfamily ribonuclease
  expressed uniquely in placenta. Nucleic Acids Res. 30: 1169-1175, 2002.
  2. Piccoli, R., Di Gaetano, S., De Lorenzo, C., Grauso, M., Monaco, C., Spalletti-Cernia, D., Laccetti, P.,
  Cinatl, J., Matousek, J., D'Alessio, G. A dimeric mutant of human pancreatic ribonuclease with
  selective cytotoxicity toward malignant cells. Proc. Nat. Acad. Sci. 96: 7768-7773, 1999
• Storage Buffer: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
• Description: Rabbit IgG polyclonal antibody for Ribonuclease pancreatic(RNASE1) detection. Tested with WB in Human.

Target Info

• Gene Symbol: RNASE1
• Gene Full Name: ribonuclease A family member 1, pancreatic
• Alias Symbols: HP-RNase;RAC1;RIB1;RIB-1;ribonuclease 1;Ribonuclease A;ribonuclease A C1;ribonuclease pancreatic;ribonuclease, RNase A family, 1 (pancreatic);RNase 1;RNase A;RNase upI-1;RNS1.
• NCBI Gene Id: 6035
• Protein Name: Ribonuclease pancreatic
• Description of Target: Endonuclease that catalyzes the cleavage of RNA on the 3' side of pyrimidine nucleotides. Acts on single-stranded and double-stranded RNA.
• Uniprot ID: P07998
• Molecular Weight: 17644 MW