Catalog No: OKCD01109 (Formerly GWB-KBBXS5)
Size:96WELLS
Price: $725.00
SKU
OKCD01109
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Datasheets/ManualsPrintable datasheet for RNASE1 ELISA Kit (Bovine) (OKCD01109)
Product Info
Predicted Species ReactivityBos taurus|Bovine
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
serum(n=5)91-102%97-105%95-105%81-89%
EDTA plasma(n=5)98-105%88-97%88-95%83-97%
heparin plasma(n=5)78-92%96-104%81-103%91-98%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ribonuclease A (RNase A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ribonuclease A (RNase A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ribonuclease A (RNase A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Ribonuclease A (RNase A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range3.12-200ng/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
serum(n=5)85-9893
EDTA plasma(n=5)88-10292
heparin plasma(n=5)89-9793
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ribonuclease A (RNase A) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ribonuclease A (RNase A) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-RNASE1 Microplate96 Wells (12 x 8 Well Strips)
RNASE1 Lyophilized Standard2 x 200 ng
100X Biotinylated RNASE1 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates and other biological fluids
Sensitivity< 1.34 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Ribonuclease A (RNASE1). No significant cross-reactivity or interference between Ribonuclease A (RNASE1) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolRNASE1
Gene Full Nameribonuclease, RNase A family, 1 (pancreatic)
Alias Symbolsribonuclease A (pancreatic);ribonuclease pancreatic;RNase 1;RNase A;RNS1.
NCBI Gene Id282340
Protein NameRibonuclease pancreatic
Description of TargetEndonuclease that catalyzes the cleavage of RNA on the 3' side of pyrimidine nucleotides. Acts on single-stranded and double-stranded RNA.
Uniprot IDP61823
Protein Accession #NP_002924
Nucleotide Accession #NM_002933.4
Protein Size (# AA)150
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