RLN2 ELISA Kit (Human) (OKEH07620)

Catalog No: OKEH07620

Size:96T

Human RLN2 ELISA Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  serum(n=5)	80-90% (n = 20)	97-105% (n = 20)	109-119% (n = 20)	99-109% (n = 20)
  EDTA plasma(n=5)	81-91% (n = 20)	104-113% (n = 20)	107-117% (n = 20)	84-92% (n = 20)
  heparin plasma(n=5)	80-92% (n = 20)	98-108% (n = 20)	107-116% (n = 20)	93-103% (n = 20)

• ELISA Kit Principle: Aviva Systems Biology RLN2 ELISA Kit (Human) (OKEH07620) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for RLN2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for RLN2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample RLN2 captured in well.
• ELISA Kit Range: 31.2-2000pg/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 115% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=6.9% (n = 20)
  Mean Inter-assay CV%: <=10.3% (n = 20)
• ELISA Kit Component:
  • RLN2 Microplate: 96 Wells (12 x 8 Well strips)
  • RLN2 Lyophilized Standard: 2
  • 100X Biotinylated RLN2 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Function: Relaxin is an ovarian hormone that acts with estrogen to produce dilatation of the birth canal in many mammals. May be involved in remodeling of connective tissues during pregnancy, promoting growth of pubic ligaments and ripening of the cervix.
  Subcellular Location: Secreted
  Unigene: Hs.127032
  SMR: P04090
  String: 9606.ENSP00000371040
  KEGG: hsa:6019
  MIM: 179740
  Pfam: PF00049
• Reconstitution and Storage: Store as indicated in product manual.
• Sensitivity: 10pg/ml
• Specificity: Natural and recombinant Human Prorelaxin H2
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: RLN2
• Gene Full Name: relaxin 2
• Alias Symbols: bA12D24.1.1;bA12D24.1.2;H2;H2-preprorelaxin;H2-RLX;prorelaxin H2;relaxin H2;relaxin, ovarian, of pregnancy;RLXH2.
• NCBI Gene Id: 6019
• Protein Name: P04090
• Description of Target: Prorelaxin H2
• Uniprot ID: https://www.uniprot.org/uniprot/P04090
• Protein Accession #: NP_005050.2
• Nucleotide Accession #: NM_005059.3