RIPK3 ELISA Kit (Human) (OKCA02138)

Catalog No: OKCA02138

Size:96 Wells

Human RIPK3 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Human
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3.5 Hours
• ELISA Kit Linearity:

  Sample Type	Dilution	Mean (%)	Range (%)
  Serum (n=4)	1:5	104	94-110
  Serum (n=4)	1:10	99	96-106
  Serum (n=4)	1:20	88	80-94
  Serum (n=4)	1:40	98	91-103

• ELISA Kit Principle: Aviva Systems Biology RIPK3 ELISA Kit (Human) (OKCA02138) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for RIPK3 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for RIPK3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is read by absorbance at 450 nm and is quantitatively proportional to the amount of sample RIPK3 captured in well.
• ELISA Kit Range: 15.6 pg/mL-1,000 pg/mL
• ELISA Kit Recovery:

  Sample Type	Mean (%)	Range (%)
  Serum (n=5)	93	89-96
  EDTA plasma (n=4)	98	90-102

• ELISA Kit Reproducibility: Mean Intra-Assay Variation <10% CV (n=20)
  Mean Inter-Assay Variation <12% CV (n=8)
• ELISA Kit Component:
  • Anti-RIPK3 Microplate: 96 Wells (12 x 8 Well strips)
  • RIPK3 Lyophilized Standard: 2 x 1 ng
  • 100X Biotinylated RIPK3 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 50 mL
  • Detector Antibody Diluent: 1 x 15 mL
  • Conjugate Diluent: 1 x 15 mL
  • 25X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 10 mL
  • TMB Substrate: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates, Cell Lysates
• Sensitivity: 3.9 pg/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: RIPK3
• Alias Symbols: RIP3
• NCBI Gene Id: 11035
• Protein Name: Receptor-interacting serine/threonine-protein kinase 3
• Description of Target: Essential for necroptosis, a programmed cell death process in response to death-inducing TNF-alpha family members. Upon induction of necrosis, RIPK3 interacts with, and phosphorylates RIPK1 and MLKL to form a necrosis-inducing complex. RIPK3 binds to and enhances the activity of three metabolic enzymes: GLUL, GLUD1, and PYGL. These metabolic enzymes may eventually stimulate the tricarboxylic acid cycle and oxidative phosphorylation, which could result in enhanced ROS production.
• Uniprot ID: Q9Y572
• Protein Accession #: NP_006862
• Nucleotide Accession #: NM_006871