RIPK3 Antibody - middle region (OAAB16957)

Catalog No: OAAB16957

Size:400ul

Western blot analysis of RIP3 (RIPK3) Antibody (Center) (Cat.# OAAB16957) in Hela cell line lysates (35ug/lane). RIPK3 (arrow) was detected using the purified Pab.

Western blot analysis of RIP3 (RIPK3) Antibody (Center) (Cat.# OAAB16957) in mouse cerebellum tissue lysates (35ug/lane). RIPK3 (arrow) was detected using the purified Pab.

Western blot analysis of lysates from HT-29, K562 cell line (from left to right), using RIPK3 Antibody Center(Cat. # OAAB16957). OAAB16957 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 35ug per lane.

Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with RIP3 (RIPK3) antibody (Center) (Cat.# OAAB16957), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Product Info

• Predicted Species Reactivity: Human, Mouse
• Product Format: Liquid. PBS with 0.09% (W/V) sodium azide
• Clonality: Polyclonal
• Isotype: Ig
• Host: Rabbit
• Application: WB, IHC-P, E
• Additional Information: Function: Essential for necroptosis, a programmed cell death process in response to death-inducing TNF-alpha family members. Upon induction of necrosis, RIPK3 interacts with, and phosphorylates RIPK1 and MLKL to form a necrosis-inducing complex. RIPK3 binds to and enhances the activity of three metabolic enzymes: GLUL, GLUD1, and PYGL. These metabolic enzymes may eventually stimulate the tricarboxylic acid cycle and oxidative phosphorylation, which could result in enhanced ROS production.
  Cellular Location: Cytoplasm, cytosol. Cell membrane Mitochondrion
  Tissue Location: Highly expressed in the pancreas. Detected at lower levels in heart, placenta, lung and kidney. Isoform 3 is significantly increased in colon and lung cancers
• Reconstitution and Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This RIPK3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 61-91 amino acids from the Central region of human RIPK3.
• Purification: This antibody is purified by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Concentration: 2 mg/ml
• Intended Use: For research use only and not for use in diagnostic or therapeutic procedures.
• Application Info: WB: 1:1,000
  IHC-P: 1:10~50
  Optimal dilutions should be determined by the end user.
• Reference: Yu P.W., Huang B.C.B., Shen M., Quast J., Chan E., Xu X., Nolan G.P., Payan D.G., Luo Y. Curr. Biol. 9:539-542(1999).
  Sun X., Lee J., Navas T., Baldwin D.T., Stewart T.A., Dixit V.M.; J. Biol. Chem. 274:16871-16875(1999).

Target Info

• Gene Symbol: RIPK3
• Alias Symbols: RIP3
• NCBI Gene Id: 11035
• Protein Name: Receptor-interacting serine/threonine-protein kinase 3
• Description of Target: The product of this gene is a member of the receptor-interacting protein (RIP) family of serine/threonine protein kinases, and contains a C-terminal domain unique from other RIP family members. The encoded protein is predominantly localized to the cytoplasm, and can undergo nucleocytoplasmic shuttling dependent on novel nuclear localization and export signals. It is a component of the tumor necrosis factor (TNF) receptor-I signaling complex, and can induce apoptosis and weakly activate the NF-kappaB transcription factor.
• Uniprot ID: Q9Y572
• Protein Accession #: NP_006862.2
• Molecular Weight: 57 kDa