RIPK2 Antibody - C-terminal region (OAAB17272)

Catalog No: OAAB17272

Size:400ul

Western blot analysis of anti-RIPK2 Pab (Cat. # OAAB17272) in mouse cerebellum tissue lysate. RIPK2 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.

Western blot analysis of anti-RIPK2 Pab (Cat. # OAAB17272) in Ramos cell line lysates (35ug/lane). RIPK2 (arrow) was detected using the purified Pab.

Western blot analysis of RIPK2 (arrow) using rabbit polyclonal RIPK2 Antibody (D474) (Cat.# OAAB17272). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the RIPK2 gene.

Product Info

• Predicted Species Reactivity: Human, Mouse
• Product Format: Liquid. PBS with 0.09% (W/V) sodium azide.
• Clonality: Polyclonal
• Isotype: Ig
• Host: Rabbit
• Application: WB, IHC-P
  Function: Serine/threonine/tyrosine kinase that plays an essential role in modulation of innate and adaptive immune responses. Upon stimulation by bacterial peptidoglycans, NOD1 and NOD2 are activated, oligomerize and recruit RIPK2 through CARD-CARD domains. Once recruited, RIPK2 autophosphorylates and undergoes 'Lys-63'-linked polyubiquitination by E3 ubiquitin ligases BIRC2 and BIRC3. The polyubiquitinated protein mediates the recruitment of MAP3K7/TAK1 to IKBKG/NEMO and induces 'Lys-63'-linked polyubiquitination of IKBKG/NEMO and subsequent activation of IKBKB/IKKB. In turn, NF-kappa-B is released from NF-kappa-B inhibitors and translocates into the nucleus where it activates the transcription of hundreds of genes involved in immune response, growth control, or protection against apoptosis. Plays also a role during engagement of the T-cell receptor (TCR) in promoting BCL10 phosphorylation and subsequent NF-kappa-B activation.
  Cellular Location: Cytoplasm (Probable).
  Tissue Location: Detected in heart, brain, placenta, lung, peripheral blood leukocytes, spleen, kidney, testis, prostate, pancreas and lymph node.
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This RIPK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 458-488 amino acids from the C-terminal region of human RIPK2.
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Predicted Homology Based on Immunogen Sequence: Human; Mouse
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Application Info: WB: 1:1000
  IHC-P: 1:50~100

Target Info

• Gene Symbol: RIPK2
• Alias Symbols: CCK, RICK, RIP2, CARD3, GIG30, CARDIAK
• NCBI Gene Id: 8767
• Protein Name: Receptor-interacting serine/threonine-protein kinase 2
• Description of Target: RIPK2 is a member of the receptor-interacting protein (RIP) family of serine/threonine protein kinases. The encoded protein contains a C-terminal recruitment domain (CARD), and is a component of signaling complexes in both the innate and adaptive immune pathways. It is a potent activator of NF-kappaB and inducer of apoptosis in response to various stimuli.
• Uniprot ID: O43353
• Protein Accession #: NP_003812.1
• Molecular Weight: 61195 Da