RHPN2 Antibody (OAEB00429)

Catalog No: OAEB00429 (Formerly GWB-D3009F)

Size:100UG

OAEB00429 (5ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Info

• Predicted Species Reactivity: Bovine|Human|Mouse|Rat
• Product Format: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Clonality: Polyclonal
• Host: Goat
• Application: IHC
• Reconstitution and Storage: Aliquot and store at -20C. Minimize freezing and thawing.
• Immunogen: RHPN2
• Purification: Ammonium Sulfate Precipitated; Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Cow: 100%; Horse: 100%; Human: 100%; Mouse: 90%; Rabbit: 100%; Rat: 90%
• Peptide Sequence: C-TNKNRQKSAST
• Concentration: 0.5 mg/ml
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Application Info: Peptide ELISA: antibody detection limit dilution 1:32000.
  Western Blot: Preliminary experiments gave an approx 35kDa band in Human Pancreas and Prostate lysates after 0.3ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 77kDa according to NP_149094.2. The 35kDa band was successfully blocked by incubation with the immunizing peptide.
• Reference: * Peck JW, Oberst M, Bouker KB, Bowden E, Burbelo PD.; The RhoA-binding protein, rhophilin-2, regulates actin cytoskeleton organization.; J Biol Chem. 2002 Nov 15;277(46):43924-32.; Pubmed 12221077

Target Info

• Gene Symbol: RHPN2
• Gene Full Name: rhophilin Rho GTPase binding protein 2
• Alias Symbols: 76 kDa RhoB effector protein;GTP-Rho-binding protein 2;P76RBE;RHOBP;rhophilin-2;rhophilin-like Rho-GTPase binding protein.
• NCBI Gene Id: 85415
• Protein Name: Rhophilin-2
• Uniprot ID: Q8IUC4
• Protein Accession #: NP_149094.3
• Molecular Weight: 77