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Rhodopsin Antibody (OASE00117)

25ug
$209.00
In Stock
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Gene Symbol:
RHO
NCBI Gene Id:
6010
Official Gene Full Name:
rhodopsin
Swissprot Id:
P08100
Protein Accession #:
NP_000530.1
Replacement Item:
This antibody may replace item sc-14355 from Santa Cruz Biotechnology.
Host:
Mouse
Clonality:
Monoclonal
Purification:
Protein G Purified
Application:
ICC, IHC, IP, WB, ELISA, IF
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express RHO.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express RHO.
Immunogen:
Bovine Rhodopsin
Predicted Species Reactivity:
Vertebrates
Reconstitution and Storage:
Store at -20C. Shipping in Blue Ice or 4C.
Concentration:
1mg/mL
Datasheets/Manuals:
Printable datasheet for OASE00117
Dilution:
1:1000 (WB)
Clone:
1D4
Isotype:
IgG1
Additional Information:
Background Info: ~40kDa.
:::
Scientific Background: Rhodopsin consists of the protein moiety opsin and a reversibly covalently bound cofactor, retinal. Opsin, a bundle of seven membrane embedded alpha-helices, binds retinal, a photo reactive chromophore, in a central pocket (2, 3). In addition to being the pigment of the retina that is responsible for both the formation of the photoreceptor cells, its function is to specifically convey information stored in the specific geometry of the chormophore to the surface of the molecule upon light absorption (2). In the active state, rhodopsin activates transduction, a GTP binding protein. Once activated, transduction promotes the hydrolysis of cGMP by phosphodiesterase. Rhodopsin's activity is believed to be shut off by its phosphorylation followed by binding of the soluble protein arrestin (4).
Mutations in the rhodopsin gene lead to retinitis pigmentosa, which can be inherited as an autosomal dominant, an autosomal recessive or an X-linked recessive disorder (5).
:::
Certificate of Analysis: 1 ug/mL was sufficient for detection of rhodopsin in 10ug of rat eye lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Storage Buffer:
PBS pH7.4, 50% glycerol
Target Reference:
1. Molday R.S., Hicks D., and Molday L. (1987) Invest Ophthalmol Vis Sci. 28: 50-61.
2. Ridge K.D., Lee S.S.J., and Abdulaev N.G. (1996) J of Biol Chem. 271: 7860-7867.
3. Matsuyama T., Yamashita T., Imai H. and Shichida Y. (2009) J Biol Chem. Manuscript M109.063875.
4. Feurstein S.E., et al. (2009) Biochemistry. 48(45): 10733-10742.
5. Iannaccone A., et al. (2006) Vision Res. 46(27): 4556-4567.

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