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Catalog No: OASE00115 (Formerly GWB-JDA220)
Size:25ug
Price: $239.00
SKU
OASE00115
Availability: Discontinued
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Rhodopsin Antibody (OASE00115)

Datasheets/ManualsPrintable datasheet for OASE00115
Product Info
Predicted Species ReactivityMammals, Fish, Avian, Amphibians, Shark
ClonalityMonoclonal
Clone4D2
IsotypeIgG1
HostMouse
ConjugationUnconjugated
ApplicationWB, IHC, ICC, IF, IP, ELISA
Additional InformationBackground Info: N-terminus of Rhodopsin
::Scientific Background: Rhodopsin consists of the protein moiety opsin and a reversibly covalently bound cofactor, retinal. Opsin, a bundle of seven membrane embedded alpha-helices, binds retinal, a photo reactive chromophore, in a central pocket (2, 3). In addition to being the pigment of the retina that is responsible for both the formation of the photoreceptor cells, its function is to specifically convey information stored in the specific geometry of the chormophore to the surface of the molecule upon light absorption (2). In the active state, rhodopsin activates transduction, a GTP binding protein. Once activated, transduction promotes the hydrolysis of cGMP by phosphodiesterase. Rhodopsin's activity is believed to be shut off by its phosphorylation followed by binding of the soluble protein arrestin (4).
Mutations in the rhodopsin gene lead to retinitis pigmentosa, which can be inherited as an autosomal dominant, an autosomal recessive or an X-linked recessive disorder (5).
::Certificate of Analysis: 1 ug/mL was sufficient for detection of rhodopsin in 10ug of rat eye lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Reconstitution and StorageStore at -20C. Shipping with Blue Ice or 4C.
ImmunogenBovine Rhodopsin
PurificationProtein G Purified
Concentration1 mg/ml
SpecificityDetects ~40kDa. Binds specifically to the N-terminus of Rhodopsin. Does not detect Rhodopsin in invertebrates.
DilutionWB (1:1000), IHC (1000); optimal dilutions for assays should be determined by the user.
Storage BufferPBS pH7.4, 50% glycerol, 0.09% sodium azide
Reference1. Molday R.S., Hicks D., and Molday L. (1987) Invest Ophthalmol Vis Sci. 28: 50-61.
2. Ridge K.D., Lee S.S.J., and Abdulaev N.G. (1996) J of Biol Chem. 271: 7860-7867.
3. Matsuyama T., Yamashita T., Imai H. and Shichida Y. (2009) J Biol Chem. Manuscript M109.063875.
4. Feurstein S.E., et al. (2009) Biochemistry. 48(45): 10733-10742.
5. Iannaccone A., et al. (2006) Vision Res. 46(27): 4556-4567.
DescriptionRhodopsin consists of the protein moiety opsin and a reversibly covalently bound cofactor, retinal. Opsin, a bundle of seven membrane embedded alpha-helices, binds retinal, a photo reactive chromophore, in a central pocket (2, 3). In addition to being the pigment of the retina that is responsible for both the formation of the photoreceptor cells, its function is to specifically convey information stored in the specific geometry of the chormophore to the surface of the molecule upon light absorption (2). In the active state, rhodopsin activates transduction, a GTP binding protein. Once activated, transduction promotes the hydrolysis of cGMP by phosphodiesterase. Rhodopsin's activity is believed to be shut off by its phosphorylation followed by binding of the soluble protein arrestin (4). Mutations in the rhodopsin gene lead to retinitis pigmentosa, which can be inherited as an autosomal dominant, an autosomal recessive or an X-linked recessive disorder (5).
Gene SymbolRhodopsin
Gene Full Namerhodopsin
Alias SymbolsOPN2, opsd, opsin 2, opsin 2 rod pigment, opsin2, RHO, RP4, MGC138309, Retinitis Pigmentosa 4
NCBI Gene Id509933
Uniprot IDP02699
Protein Accession #NP_001014890.1
  1. What is the species homology for "Rhodopsin Antibody (OASE00115)"?

    The tested species reactivity for this item is "". This antibody is predicted to have homology to "Mammals, Fish, Avian, Amphibians, Shark".

  2. How long will it take to receive "Rhodopsin Antibody (OASE00115)"?

    This item is available "Domestic: within 1-2 weeks delivery | International: 1-2 weeks".

  3. What buffer format is "Rhodopsin Antibody (OASE00115)" provided in?

    This item is provided in "".
    Additional format options may be available. For more information please contact info@avivasysbio.com.

  4. What are other names for "Rhodopsin Antibody (OASE00115)"?

    This target may also be called "OPN2, opsd, opsin 2, opsin 2 rod pigment, opsin2, RHO, RP4, MGC138309, Retinitis Pigmentosa 4" in publications.

  5. What is the shipping cost for "Rhodopsin Antibody (OASE00115)"?

    The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.

  6. What is the guarantee for "Rhodopsin Antibody (OASE00115)"?

    All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.

  7. Can I get bulk pricing for "Rhodopsin Antibody (OASE00115)"?

    You can get bulk pricing for this item by going here.

  8. What is the molecular weight of the protein?

    The molecular weight reported by Uniprot for this item is "".
    Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics.

  9. What protocols are available for "Rhodopsin Antibody (OASE00115)"?

    We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.

  10. What are positive controls for "Rhodopsin"?

    We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.

  11. What are negative controls for "Rhodopsin"?

    We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.

  12. What other proteins interact with "Rhodopsin"?

    This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.

  13. What biological processes are associated with "Rhodopsin"?

    This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.

  14. What cellular components are associated with "Rhodopsin"?

    This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.

  15. What protein functions are associated with "Rhodopsin"?

    This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.

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