- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
Datasheets/Manuals | Printable datasheet for OASE00116-HRP |
---|
Predicted Species Reactivity | Human, Mouse, Rat, Cow, Pig |
---|---|
Product Format | Liquid. Purified antibody is supplied in high phosphate PBS, 100 mm phosphate, 150 mM NaCl, pH 7.6. |
Clonality | Monoclonal |
Clone | 4D2 |
Isotype | IgG1 |
Host | Mouse |
Conjugation | HRP: Horseradish Peroxidase |
Application | ICC, IHC, IP, WB, ELISA |
Additional Information | Background Info: N-terminus of Rhodopsin |
:: | Scientific Background: Rhodopsin consists of the protein moiety opsin and a reversibly covalently bound cofactor, retinal. Opsin, a bundle of seven membrane embedded alpha-helices, binds retinal, a photo reactive chromophore, in a central pocket (2, 3). In addition to being the pigment of the retina that is responsible for both the formation of the photoreceptor cells, its function is to specifically convey information stored in the specific geometry of the chormophore to the surface of the molecule upon light absorption (2). In the active state, rhodopsin activates transduction, a GTP binding protein. Once activated, transduction promotes the hydrolysis of cGMP by phosphodiesterase. Rhodopsin's activity is believed to be shut off by its phosphorylation followed by binding of the soluble protein arrestin (4). Mutations in the rhodopsin gene lead to retinitis pigmentosa, which can be inherited as an autosomal dominant, an autosomal recessive or an X-linked recessive disorder (5). |
:: | Certificate of Analysis: 1 ug/mL was sufficient for detection of rhodopsin in 10ug of rat eye lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. |
Reconstitution and Storage | All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil). Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding. |
Immunogen | Bovine Rhodopsin |
Purification | Protein G Purified |
Concentration | 0.6-0.7 mg/ml |
Dilution | 1:1000 (WB) |
Storage Buffer | PBS pH7.4, 50% glycerol |
Reference | 1. Molday R.S., Hicks D., and Molday L. (1987) Invest Ophthalmol Vis Sci. 28: 50-61. 2. Ridge K.D., Lee S.S.J., and Abdulaev N.G. (1996) J of Biol Chem. 271: 7860-7867. 3. Matsuyama T., Yamashita T., Imai H. and Shichida Y. (2009) J Biol Chem. Manuscript M109.063875. 4. Feurstein S.E., et al. (2009) Biochemistry. 48(45): 10733-10742. 5. Iannaccone A., et al. (2006) Vision Res. 46(27): 4556-4567. |
Gene Symbol | RHO |
---|---|
Gene Full Name | rhodopsin |
Alias Symbols | RP4, OPN2, CSNBAD1 |
NCBI Gene Id | 6010 |
Uniprot ID | P08100 |
Protein Accession # | NP_000530.1 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
-
What is the species homology for "Rhodopsin Antibody : HRP (OASE00116-HRP)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human, Mouse, Rat, Cow, Pig".
-
How long will it take to receive "Rhodopsin Antibody : HRP (OASE00116-HRP)"?
This item is available "Domestic: within 3-5 days delivery | International: 3-5 days".
-
What buffer format is "Rhodopsin Antibody : HRP (OASE00116-HRP)" provided in?
This item is provided in "Liquid. Purified antibody is supplied in high phosphate PBS, 100 mm phosphate, 150 mM NaCl, pH 7.6.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "Rhodopsin Antibody : HRP (OASE00116-HRP)"?
This target may also be called "RP4, OPN2, CSNBAD1" in publications.
-
What is the shipping cost for "Rhodopsin Antibody : HRP (OASE00116-HRP)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
-
What is the guarantee for "Rhodopsin Antibody : HRP (OASE00116-HRP)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
-
Can I get bulk pricing for "Rhodopsin Antibody : HRP (OASE00116-HRP)"?
You can get bulk pricing for this item by going here.
-
What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "Rhodopsin Antibody : HRP (OASE00116-HRP)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
-
What are positive controls for "RHO"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
-
What are negative controls for "RHO"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
-
What other proteins interact with "RHO"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
-
What biological processes are associated with "RHO"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
-
What cellular components are associated with "RHO"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
-
What protein functions are associated with "RHO"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.