RHOD Antibody - C-terminal region (ARP42414_P050)

Catalog#: ARP42414_P050

• Size: 100 ul

• Tested Species Reactivity: Human
• Predicted Species Reactivity: Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rat
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Clonality: Polyclonal
• Host: Rabbit
• Application: WB, IHC
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Replacement Item: This antibody may replace item sc-27879 from Santa Cruz Biotechnology.
• Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human RHOD
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 92%; Human: 100%; Mouse: 82%; Rat: 100%
• Peptide Sequence: Synthetic peptide located within the following region: NGLEPVTYHRGQEMARSVGAVAYLECSARLHDNVHAVFQEAAEVALSSRG
• Concentration: Batch dependent within range: 100 ul at 0.5 - 1 mg/ml
• Blocking Peptide: For anti-RHOD (ARP42414_P050) antibody is Catalog # AAP42414 (Previous Catalog # AAPP24760)
• Target Reference: Tong,Y., (2007) J. Biol. Chem. 282 (51), 37215-37224
• Gene Symbol: RHOD
• Official Gene Full Name: Ras homolog family member D
• Alias Symbols: ARHD, RHOHP1, RHOM, Rho
• NCBI Gene Id: 29984
• Protein Name: Rho-related GTP-binding protein RhoD
• Description of Target: Ras homolog, or Rho, proteins interact with protein kinases and may serve as targets for activated GTPase. They play a critical role in muscle differentiation. RHOD binds GTP and is a member of the small GTPase superfamily. It is involved in endosome dynamics and reorganization of the actin cytoskeleton, and it may coordinate membrane transport with the function of the cytoskeleton. Ras homolog, or Rho, proteins interact with protein kinases and may serve as targets for activated GTPase. They play a critical role in muscle differentiation. The protein encoded by this gene binds GTP and is a member of the small GTPase superfamily. It is involved in endosome dynamics and reorganization of the actin cytoskeleton, and it may coordinate membrane transport with the function of the cytoskeleton. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
• Swissprot Id: O00212
• Protein Accession #: NP_055393
• Nucleotide Accession #: NM_014578
• Protein Size (# AA): 210
• Molecular Weight: 23kDa
• RNA Seq: Find tissues and cell lines supported by RNA-seq analysis to express RHOD.
• Protein Interactions: ADRB2; IHH; ADCK5; UBC; PLXNA1; SMURF2; BMPR1B; SMAD2; SMAD4; DIAPH2; TGFBR1; HRAS; ACVR1; CNKSR1; ARHGAP29;

Protocol:

• Reconstitution Instructions
• Immunohistochemistry (IHC) Protocol
• Immunocytochemistry (ICC) Protocol
• Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
• Western Blotting/Immunoblotting (WB/IB) Protocol
• Blocking Peptide Competition Protocol (BPCP)
• Immunoprecipitation (IP) Protocol

Tips Information:

• IHC Tips & Tricks
• ICC Tips & Tricks
• ELISA Tips & Tricks
• WB/IB Tips & Tricks
• IP Tips & Tricks

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Product Protocols: RHOD antibody tested with Human Transfected 293T Cells (ARP42414_P050)

Product Protocols: RHOD antibody tested by IHC with Human Liver Tissue ARP42414_P050

Product Protocols: RHOD antibody tested by IHC with Human Bronchial Epithelial Tissue ARP42414_P050