RHOC ELISA Kit (Chicken) (OKEH07683)

Catalog No: OKEH07683

Size:96T

Product Info

• Predicted Species Reactivity: Chicken, Gallus gallus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Principle: Aviva Systems Biology RHOC ELISA Kit (Chicken) (OKEH07683) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for RHOC has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for RHOC is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample RHOC captured in well.
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 95% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=3.7% (n = 20)
  Mean Inter-assay CV%: <=12.3% (n = 20)
• ELISA Kit Component:
  • RHOC Microplate: 96 Wells (12 x 8 Well strips)
  • RHOC Lyophilized Standard: 2
  • 100X Biotinylated RHOC Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Function: Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. Serves as a microtubule-dependent signal that is required for the myosin contractile ring formation during cell cycle cytokinesis. Regulates apical junction formation in bronchial epithelial cells.
  Subcellular Location: Cell membrane Lipid-anchor Cytoplasmic side Cleavage furrow Translocates to the equatorial region before furrow formation in a ECT2-dependent manner.
  Unigene: Gga.17535
  SMR: Q9PSX7
  String: 9031.ENSGALP00000039370
  KEGG: gga:395869
  Pfam: PF00071
• Reconstitution and Storage: Store as indicated in product manual.
• Specificity: Natural and recombinant Chicken Rho-related GTP-binding protein RhoC
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: RHOC
• Gene Full Name: ras homolog family member C
• Alias Symbols: CRHOC;GTPase cRhoC;GTP-binding protein;ras homolog gene family, member C;rho-related GTP-binding protein RhoC.
• NCBI Gene Id: 395869
• Protein Name: Q9PSX7
• Description of Target: Rho-related GTP-binding protein RhoC
• Uniprot ID: https://www.uniprot.org/uniprot/Q9PSX7
• Protein Accession #: NP_001025020.1
• Nucleotide Accession #: NM_001029849.1