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Catalog No: OCOA00562
Size:20UG
Price: $179.00
SKU
OCOA00562
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RHOA Protein Lysate (OCOA00562)

Datasheets/ManualsPrintable datasheet for RHOA Protein Lysate (OCOA00562)
Product Info
Predicted Species ReactivityHomo sapiens
Product FormatLyophilized
HostHEK293T
Additional InformationComponents: 1 vial of 20 ug lyophilized gene specific transient over-expression cell lysate
::Preparation: HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution and StorageThe lysate can be shipped at ambient temperature. Upon receiving, store the sample at -20C. Lysate samples are stable for 12 months from date of receipt when stored at -20C. Avoid repeated freeze-thaw cycles after reconstitution. Lysate samples can be reconstituted with SDS Sample Buffer. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes
TagC-MYC/DDK
DescriptionRHOA HEK293T cell transient overexpression lysate (as WB positive control)
Gene SymbolRHOA
Gene Full Nameras homolog family member A
Alias SymbolsAplysia ras-related homolog 12;ARH12;ARHA;EDFAOB;epididymis secretory sperm binding protein;oncogene RHO H12;Rho cDNA clone 12;RHO12;RHOH12;small GTP binding protein RhoA;transforming protein RhoA.
NCBI Gene Id387
Protein NameA0A024R324, NM_001664, NP_001655, P61586
Description of TargetEpididymis secretory sperm binding protein, Homo sapiens ras homolog family member A (RHOA), transcript variant 1, mRNA, Transforming protein RhoA, transforming protein RhoA isoform 1 precursor [Homo sapiens]
Uniprot IDhttps://www.uniprot.org/uniprot/P61586
Protein Accession #https://www.ncbi.nlm.nih.gov/protein/NP_001655
Nucleotide Accession #https://www.ncbi.nlm.nih.gov/nuccore/NM_001664
Molecular Weight21.8 kDa
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