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Catalog No: OKCD02814
Size:96WELLS
Price: $725.00
SKU
OKCD02814
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Datasheets/ManualsPrintable datasheet for RHOA ELISA Kit (Rat) (OKCD02814)
Product Info
Predicted Species ReactivityRat|Rattus norvegicus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ras Homolog Gene Family, Member A (RHOA). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ras Homolog Gene Family, Member A (RHOA). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ras Homolog Gene Family, Member A (RHOA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Ras Homolog Gene Family, Member A (RHOA) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ras Homolog Gene Family, Member A (RHOA) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ras Homolog Gene Family, Member A (RHOA) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-RHOA Microplate96 Wells (12 x 8 Well strips)
RHOA Lyophilized Standard2
100X Biotinylated RHOA Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
Stop Solution1 x 6 mL
TMB Substrate1 x 9 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates, cell lysates and other biological fluids
Sensitivity0.065 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Ras Homolog Gene Family, Member A (RHOA). No significant cross-reactivity or interference between Ras Homolog Gene Family, Member A (RHOA) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Protocol Information
Gene SymbolRhoa
Gene Full Nameras homolog family member A
Alias SymbolsArha;Arha2;plysia ras-related homolog A2;ras homolog gene family, member A;transforming protein RhoA.
NCBI Gene Id117273
Protein NameTransforming protein RhoA
Description of TargetRegulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. Involved in a microtubule-dependent signal that is required for the myosin contractile ring formation during cell cycle cytokinesis. Plays an essential role in cleavage furrow formation. Required for the apical junction formation of keratinocyte cell-cell adhesion. May be an activator of PLCE1. Activated by ARHGEF2, which promotes the exchange of GDP for GTP. Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly. The MEMO1-RHOA-DIAPH1 signaling pathway plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. It controls the localization of APC and CLASP2 to the cell membrane, via the regulation of GSK3B activity. In turn, membrane-bound APC allows the localization of the MACF1 to the cell membrane, which is required for microtubule capture and stabilization (By similarity). Regulates KCNA2 potassium channel activity by reducing its location at the cell surface in response to CHRM1 activation; promotes KCNA2 endocytosis (PubMed:9635436).
Uniprot IDP61589
Protein Accession #NP_476473.1
Nucleotide Accession #NM_057132.3
Protein Size (# AA)193
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