RHO ELISA Kit (Pig) (OKEH08124)

Catalog No: OKEH08124

Size:96T

Pig RHO ELISA Standard Curve

Product Info

• Predicted Species Reactivity: Porcine, Sus scrofa
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  serum(n=5)	107-117% (n = 20)	109-118% (n = 20)	110-119% (n = 20)	92-102% (n = 20)
  EDTA plasma(n=5)	100-111% (n = 20)	113-125% (n = 20)	83-94% (n = 20)	105-117% (n = 20)
  heparin plasma(n=5)	96-106% (n = 20)	108-120% (n = 20)	100-110% (n = 20)	90-100% (n = 20)

• ELISA Kit Principle: Aviva Systems Biology RHO ELISA Kit (Pig) (OKEH08124) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for RHO has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for RHO is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample RHO captured in well.
• ELISA Kit Range: 78-5000pg/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 111% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=6.8% (n = 20)
  Mean Inter-assay CV%: <=10.4% (n = 20)
• ELISA Kit Component:
  • RHO Microplate: 96 Wells (12 x 8 Well strips)
  • RHO Lyophilized Standard: 2
  • 100X Biotinylated RHO Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Function: Photoreceptor required for image-forming vision at low light intensity. Required for photoreceptor cell viability after birth. Light-induced isomerization of 11-cis to all-trans retinal triggers a conformational change leading to G-protein activation and release of all-trans retinal.
  Subcellular Location: Membrane Multi-pass membrane protein Synthesized in the inner segment (IS) of rod photoreceptor cells before vectorial transport to the rod outer segment (OS) photosensory cilia.
  Unigene: Ssc.16150
  String: 9823.ENSSSCP00000012353
  KEGG: ssc:397437
  Pfam: PF00001
• Reconstitution and Storage: Store as indicated in product manual.
• Sensitivity: 20pg/mL
• Specificity: Natural and recombinant Pig Rhodopsin
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: RHO
• Gene Full Name: rhodopsin
• Alias Symbols: PIGRHO1;rhodopsin.
• NCBI Gene Id: 397437
• Protein Name: O18766
• Description of Target: Rhodopsin
• Uniprot ID: https://www.uniprot.org/uniprot/O18766
• Protein Accession #: NP_999386.1
• Nucleotide Accession #: NM_214221.1