RGN ELISA Kit (Chicken) (OKEH08812)

Catalog No: OKEH08812

Size:96T

Product Info

• Predicted Species Reactivity: Chicken, Gallus gallus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Principle: Aviva Systems Biology RGN ELISA Kit (Chicken) (OKEH08812) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for RGN has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for RGN is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample RGN captured in well.
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 92% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=8.0% (n = 20)
  Mean Inter-assay CV%: <=8.2% (n = 20)
• ELISA Kit Component:
  • RGN Microplate: 96 Wells (12 x 8 Well strips)
  • RGN Lyophilized Standard: 2
  • 100X Biotinylated RGN Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Function: Gluconolactonase with low activity towards other sugar lactones, including gulonolactone and galactonolactone. Catalyzes a key step in ascorbic acid (vitamin C) biosynthesis. Can also hydrolyze diisopropyl phosphorofluoridate and phenylacetate (in vitro). Calcium-binding protein. Modulates Ca(2+) signaling, and Ca(2+)-dependent cellular processes and enzyme activities.
  Subcellular Location: Cytoplasm
  Unigene: Gga.2861
  SMR: Q9I923
  String: 9031.ENSGALP00000035812
  KEGG: gga:395480
  Pfam: PF08450
• Reconstitution and Storage: Store as indicated in product manual.
• Specificity: Natural and recombinant Chicken Regucalcin
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: RGN
• Gene Full Name: regucalcin
• Alias Symbols: gluconolactonase;GNL;RC;regucalcin.
• NCBI Gene Id: 395480
• Protein Name: Q9I923
• Description of Target: Regucalcin
• Uniprot ID: https://www.uniprot.org/uniprot/Q9I923
• Protein Accession #: NP_990060.1
• Nucleotide Accession #: NM_204729.1