RFC5 Antibody - N-terminal region (ARP46172_P050)

Catalog No: ARP46172_P050

WB Suggested Anti-RFC5 Antibody Titration: 1 ug/ml
Positive Control: Jurkat cell lysateRFC5 is strongly supported by BioGPS gene expression data to be expressed in Human Jurkat cells

Human kidney

Product Info

• Tested Species Reactivity: Human
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Yeast, Zebrafish
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Clonality: Polyclonal
• Host: Rabbit
• Application: IHC, WB
• Additional Information: IHC Information: Lane A: Marker. Lane B: Jurkat cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human RFC5
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Cow: 93%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 93%; Rat: 100%; Yeast: 90%; Zebrafish: 93%
• Peptide Sequence: Synthetic peptide located within the following region: METSALKQQEQPAATKIRNLPWVEKYRPQTLNDLISHQDILSTIQKFINE
• Concentration: 0.5 mg/ml
• Blocking Peptide: For anti-RFC5 (ARP46172_P050) antibody is Catalog # AAP46172 (Previous Catalog # AAPP27009)
• Sample Type Confirmation:

  RFC5 is strongly supported by BioGPS gene expression data to be expressed in Jurkat

• Subunit: 5
• Reference: Ohta,S., (2002) J. Biol. Chem. 277 (43), 40362-40367

Target Info

• Gene Symbol: RFC5
• Gene Full Name: Replication factor C (activator 1) 5, 36.5kDa
• Alias Symbols: RFC36
• NCBI Gene Id: 5985
• Protein Name: Replication factor C subunit 5
• Description of Target: The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kD. RFC5 is the 36 kD subunit. This subunit can interact with the C-terminal region of PCNA. It forms a core complex with the 38 and 40 kDa subunits. The core complex possesses DNA-dependent ATPase activity, which was found to be stimulated by PCNA in an in vitro system.The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kD. This gene encodes the 36 kD subunit. This subunit can interact with the C-terminal region of PCNA. It forms a core complex with the 38 and 40 kDa subunits. The core complex possesses DNA-dependent ATPase activity, which was found to be stimulated by PCNA in an in vitro system. Alternatively spliced transcript variants encoding distinct isoforms have been reported.
• Uniprot ID: P40937
• Protein Accession #: NP_031396
• Nucleotide Accession #: NM_007370
• Protein Size (# AA): 340
• Molecular Weight: 38kDa
• Protein Interactions: NAB2; XIAP; TRIM38; HUWE1; RFC4; SUMO2; PCNA; UBC; RPA3; RPA2; RPA1; EED; FBXO6; MMS19; VCP; ECT2; MDC1; MTMR2; RFC3; RFC2; RFC1; CAND1; CUL3; NEDD8; HDGF; SMARCAD1; PIDD1; MYC; RPAP3; MEPCE; RUVBL2; LRIF1; COPS6; MED31; RBM48; UNC119; EEF1A1; RAD17; BRD4