Catalog No: OAGA00277
Size:100 ul
Price: $372.00
SKU
OAGA00277
Availability: Domestic: within 1-2 week delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
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Datasheets/ManualsPrintable datasheet for anti-RFC4 antibody - OAGA00277
Product Info
Predicted Species ReactivityHuman, Mouse
Product FormatLiquid
ClonalityPolyclonal
IsotypeIgG
HostRabbit
ApplicationICC, IF, IHC-FFPE, WB
Additional InformationBackground: The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kD. This gene encodes the 37 kD subunit. This subunit forms a core complex with the 36 and 40 kDa subunits. The core complex possesses DNA-dependent ATPase activity, which was found to be stimulated by PCNA in an in vitro system. Alternatively spliced transcript variants encoding the same protein have been reported. [provided by RefSeq]
::Positive Controls: 293T, A431, H1299, HeLa, HepG2, Molt-4, Raji, mouse liver
::Cellular Localization: Nucleus
Reconstitution and StorageKeep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
ImmunogenRecombinant fragment corresponding to a region within amino acids 18 and 323 of RFC4 (Uniprot ID#P35249)
PurificationPurified by antigen-affinity chromatography.
Concentration0.62 mg/ml
Application InfoSuggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Storage Buffer0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Gene SymbolRFC4
Gene Full NameReplication factor C subunit 4
Alias SymbolsA1, RFC37
NCBI Gene Id5984
Protein NameReplication factor C subunit 4
Uniprot IDP35249
Protein Accession #NP_002907.1
Molecular Weight40 kDa
  1. What is the species homology for "RFC4 Antibody (OAGA00277)"?

    The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human, Mouse".

  2. How long will it take to receive "RFC4 Antibody (OAGA00277)"?

    This item is available "Domestic: within 1-2 week delivery | International: 1-2 weeks".

  3. What buffer format is "RFC4 Antibody (OAGA00277)" provided in?

    This item is provided in "Liquid".
    Additional format options may be available. For more information please contact info@avivasysbio.com.

  4. What are other names for "RFC4 Antibody (OAGA00277)"?

    This target may also be called "A1, RFC37" in publications.

  5. What is the shipping cost for "RFC4 Antibody (OAGA00277)"?

    The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.

  6. What is the guarantee for "RFC4 Antibody (OAGA00277)"?

    All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.

  7. Can I get bulk pricing for "RFC4 Antibody (OAGA00277)"?

    You can get bulk pricing for this item by going here.

  8. What is the molecular weight of the protein?

    The molecular weight reported by Uniprot for this item is "40 kDa".
    Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics.

  9. What protocols are available for "RFC4 Antibody (OAGA00277)"?

    We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.

  10. What are positive controls for "RFC4"?

    We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.

  11. What are negative controls for "RFC4"?

    We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.

  12. What other proteins interact with "RFC4"?

    This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.

  13. What biological processes are associated with "RFC4"?

    This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.

  14. What cellular components are associated with "RFC4"?

    This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.

  15. What protein functions are associated with "RFC4"?

    This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.

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