Catalog No: OKWB00207
Size:96T
Price: $450.00
SKU
OKWB00207
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
Contact Us:
- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
Shipping Info:
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
Datasheets/Manuals | Printable datasheet for RFC1 ELISA Kit (Human) (OKWB00207) |
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Predicted Species Reactivity | Homo sapiens|Human | ||||||||||||||||||||||
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Application | Enzyme-linked Immunosorbent assay-Sandwich | ||||||||||||||||||||||
ELISA Kit Detection Method | Colorimetric, OD 450 nm | ||||||||||||||||||||||
ELISA Kit Duration | ~ 3 Hours | ||||||||||||||||||||||
ELISA Kit Linearity |
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ELISA Kit Principle | Aviva Systems Biology RFC1 ELISA Kit (Human) (OKWB00207) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for RFC1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for RFC1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample RFC1 captured in the well. | ||||||||||||||||||||||
ELISA Kit Range | 31.25-2000pg/ml | ||||||||||||||||||||||
ELISA Kit Recovery |
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ELISA Kit Reproducibility | Mean Intra-Assay Precision <10% Mean Inter-Assay Precision <12% | ||||||||||||||||||||||
ELISA Kit Component |
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Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
Sample Type | Serum, Plasma, Cell Cultures, Tissue Homogenates, Other Biological Fluids | ||||||||||||||||||||||
Sensitivity | 18.75 pg/mL | ||||||||||||||||||||||
Assay Info | Assay Type: Quantitative Sandwich ELISA |
Gene Symbol | SLC19A1 |
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Gene Full Name | solute carrier family 19 member 1 |
Alias Symbols | CHMD;folate transporter 1;FOLT;hRFC;hSLC19A1;IFC1;IFC-1;intestinal folate carrier 1;MEGAF;placental folate transporter;reduced folate carrier 1;reduced folate carrier protein;reduced folate transporter;reduced folate transporter 1;REFC;RFC;RFC1;RFT-1;solute carrier family 19 (folate transporter), member 1;Solute carrier family 19 member 1. |
NCBI Gene Id | 6573 |
Protein Name | Folate transporter 1 |
Description of Target | Transporter for the intake of folate. Uptake of folate in human placental choriocarcinoma cells occurs by a novel mechanism called potocytosis which functionally couples three components, namely the folate receptor, the folate transporter, and a V-type H(+)-pump. |
Uniprot ID | P41440 |
Protein Accession # | NP_001191676 |
Nucleotide Accession # | NM_001204747 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
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