RETNLB ELISA Kit (Human) (OKCD01145)

Catalog No: OKCD01145 (Formerly GWB-KBBWX7)

Size:96WELLS

Human RETNLB ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  serum(n=5)	79-102%	87-96%	80-101%	90-105%
  EDTA plasma(n=5)	78-104%	79-103%	99-105%	93-105%
  heparin plasma(n=5)	85-103%	91-101%	86-94%	78-97%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Resistin Like Beta (RETNLb). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Resistin Like Beta (RETNLb). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Resistin Like Beta (RETNLb), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Resistin Like Beta (RETNLb) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 62.5-4,000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  serum(n=5)	85-93	90
  EDTA plasma(n=5)	80-96	87
  heparin plasma(n=5)	80-95	88

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Resistin Like Beta (RETNLb) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Resistin Like Beta (RETNLb) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-RETNLB Microplate: 96 Wells (12 x 8 Well Strips)
  • RETNLB Lyophilized Standard: 2 x 4 ng
  • 100X Biotinylated RETNLB Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma and other biological fluids.
• Sensitivity: < 22.5 pg/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Resistin Like Beta (RETNLb). No significant cross-reactivity or interference between Resistin Like Beta (RETNLb) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: RETNLB
• Gene Full Name: resistin like beta
• Alias Symbols: C/EBP-epsilon regulated myeloid-specific secreted cysteine-rich protein precursor 2;colon and small intestine-specific cysteine-rich protein;colon carcinoma-related gene protein;cysteine-rich secreted A12-alpha-like protein 1;cysteine-rich secreted protein A12-alpha-like 1;cysteine-rich secreted protein FIZZ2;FIZZ1;FIZZ2;found in inflammatory zone 1;HXCP2;RELMb;RELMbeta;RELM-beta;resistin-like beta;XCP2.
• NCBI Gene Id: 84666
• Protein Name: Resistin-like beta
• Description of Target: Probable hormone.
• Uniprot ID: Q9BQ08
• Protein Accession #: NP_076370
• Nucleotide Accession #: NM_023881.4
• Protein Size (# AA): 111