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Catalog No: OKCD06895
Size:96WELLS
Price: $500.00
SKU
OKCD06895
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Datasheets/ManualsPrintable datasheet for RETN ELISA Kit (Mouse) (OKCD06895)
Product Info
Predicted Species ReactivityMouse|Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Resistin (RETN). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Resistin (RETN). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Resistin (RETN), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Resistin (RETN) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.312-20ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Resistin (RETN) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Resistin (RETN) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
RETN Microplate96 Wells (12 x 8 Well strips)
RETN Lyophilized Standard2
100X RETN HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity< 0.131ng/mL
Gene SymbolRetn
Gene Full Nameresistin
Alias SymbolsAD;adipose tissue-specific secretory factor;adipose-specific cysteine-rich secreted protein A12-alpha;ADSF;cysteine-rich secreted protein FIZZ3;dominant inhibitory adipocyte-specific secretory factor;Fizz;Fizz3;found in inflammatory zone 3;r;resistin;Rstn;Xcp;Xcp4.
NCBI Gene Id57264
Protein NameResistin
Description of TargetHormone that seems to suppress insulin ability to stimulate glucose uptake into adipose cells. Potentially links obesity to diabetes.
Uniprot IDQ99P87
Protein Accession #NP_001191888.1
Nucleotide Accession #NM_001204959.1
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