Retinol Binding Protein ELISA Kit (Mouse) (OKIA00179)

Catalog No: OKIA00179

Size:1x96WELLPLATE

Sample Standard Curve is for demonstration only. Do not use for results calculations. A standard curve should be generated each time the test is performed.

Product Info

• Predicted Species Reactivity: Mouse
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: 70 Minutes
• ELISA Kit Principle: Aviva Systems Biology Retinol Binding Protein ELISA Kit (Mouse) (OKIA00179) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody for Retinol Binding Protein has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and washed to remove unbound proteins. An Anti-Retinol Binding Protein - HRP conjugated detector antibody is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Retinol Binding Protein captured in the well.
• ELISA Kit Range: 0.625 ng/ml - 20 ng/ml
• ELISA Kit Component:
  • Anti-Mouse Retinol Binding Protein Microplate: 96 Wells (12 x 8 Well Strips)
  • 20X Diluent Concentrate: 50 mL
  • 20X Wash Solution: 50 mL
  • 100X Enzyme-Antibody Conjugate: 150 uL
  • Chromogen-Substrate Solution: 12 mL
  • Stop Solution: 12 mL
  • Mouse Retinol Binding Protein Calibrator: 1
• Additional Information: Intended Use: The Retinol Binding Protein (RBP) test kit is a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring RBP in biological fluid of Mouse.
  REAGENTS
  1. DILUENT CONCENTRATE (Running Buffer)
  One bottle containing 50 ml of a 5X concentrated diluent running buffer.
  2. WASH SOLUTION CONCENTRATE
  One bottle containing 50 ml of a 20X concentrated wash solution.
  3. ENZYME-ANTIBODY CONJUGATE 100X
  One vial containing 150 L of affinity purified anti-Mouse Retinol Binding Protein antibody conjugated with horseradish peroxidase in a stabilizing buffer.
  4. CHROMOGEN-SUBSTRATE SOLUTION
  One vial containing 12 mL of 3,3’,5,5’-tetramethybenzidine (TMB) and hydrogen peroxide in citric acid buffer at pH 3.3.
  5. STOP SOLUTION
  One vial containing 12 ml 0.3 M sulfuric acid. WARNING: Avoid contact with skin.
  6. ANTI-Mouse Retinol Binding Protein ELISA MICRO PLATE
  Twelve removable eight (8) well micro well strips in well holder frame. Each well is coated with affinity purified anti-Mouse RBP.
  7. Mouse Retinol Binding Protein CALIBRATOR
  One vial containing a lyophilized Mouse Retinol Binding Protein calibrator.
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: 0.152 ng/ml
• Predicted Homology Based on Immunogen Sequence: Mouse
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Protein Name: Retinol Binding Protein