Aviva Systems Biology will be closed in observance of Good Friday 3/29/2024. If you are in need of any assistance please email info@avivasysbio.com

Catalog No: OKEH04205
Size:96T
Price: $525.00
SKU
OKEH04205
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
Please contact customer service when ordering 10+ kits.

Bulk
Order
Aviva's
Satisfaction
Guarantee
Contact Us:
Shipping Info:
  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
  • Contact us for international orders.
Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityRat, Rattus norvegicus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology Ret ELISA Kit (Rat) (OKEH04205) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Ret has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Ret is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Ret captured in the well.
ELISA Kit Range0.156-10ng/ml
ELISA Kit Component
ComponentAmount
Ret Microplate96 Wells (12 x 8 Well Strips)
Ret Standard2 x 2 ng
Sample Diluent1 x 20 mL
Ret Biotinylated Detector Antibody1 x 120 uL
Detector Antibody Diluent1 x 12 mL
HRP Avidin Conjugate1 x 120 uL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.
Sensitivity0.09 ng/mL
SpecificityNatural and recombinant Rat Proto-oncogene tyrosine-protein kinase receptor Ret
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolRet
Gene Full Nameret proto-oncogene
Alias Symbolsproto-oncogene tyrosine-protein kinase receptor Ret;receptor tyrosine kinase;Ret gene for receptor tyrosin;Ret proto-oncogene (multiple endocrine neoplasia MEN2A MEN2B and medullary thyroid carcinoma 1 Hirschsprung disease).
NCBI Gene Id24716
Protein NameG3V9H8
Description of TargetProto-oncogene tyrosine-protein kinase receptor Ret
Uniprot IDhttps://www.uniprot.org/uniprot/G3V9H8
Protein Accession #NP_036775.2
Nucleotide Accession #NM_012643.2
Protein Size (# AA)1115
Molecular Weight124 kDa
Write Your Own Review
You're reviewing:RET ELISA Kit (Rat) (OKEH04205)
Your Rating
We found other products you might like!