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Datasheets/Manuals | Printable datasheet for Resistin Polyclonal Antibody (OABB00427) |
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Tested Species Reactivity | Mouse |
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Predicted Species Reactivity | Mouse |
Product Format | Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. |
Clonality | Polyclonal |
Clone | Polyclonal |
Isotype | Rabbit IgG |
Application | Immunohistochemistry|Western blot |
Additional Information | Notes: Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. |
:: | Background: Resistin, also known as adipose tissue-specific secretory factor(ADSF) or C/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich protein(XCP1) is a cysteine-rich protein that in humans is encoded by the RETN gene. The resistin gene comprises 4 exons, the first of which is untranslated, and spans approximately 1,750 bp. The human resistin gene is localized to a cloned fragment of human chromosome 19. In primates, pigs and dogs, resistin is secreted by immune and epithelial cells while in rodents, it is secreted by adipose tissue. Resistin is a cytokine whose physiologic role has been the subject of much controversy regarding its involvement with obesity and type II diabetes mellitus. |
:: | Background: Resistin, also known as adipose tissue-specific secretory factor (ADSF) or C/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich protein (XCP1) is a cysteine-rich protein that in humans is encoded by the RETN gene. The resistin gene comprises 4 exons, the first of which is untranslated, and spans approximately 1,750 bp. The human resistin gene is localized to a cloned fragment of human chromosome 19. In primates, pigs and dogs, resistin is secreted by immune and epithelial cells while in rodents, it is secreted by adipose tissue. Resistin is a cytokine whose physiologic role has been the subject of much controversy regarding its involvement with obesity and type II diabetes mellitus. |
Reconstitution and Storage | 2°C to 8°C|-20°C |
Immunogen | Polypeptide |
Purification | Affinity Purified |
Predicted Homology Based on Immunogen Sequence | Mouse |
Concentration | 500 ug/ml |
Specificity | No cross reactivity with other proteins. |
Application Info | Western blot: 0.1-0.5 ug/ml: Mouse Immunohistochemistry (Paraffin-embedded Section): 0.5-1 ug/ml: Mouse: By Heat |
Reference | 1. Holcomb, I. N., Kabakoff, R. C., Chan, B., Baker, T. W., Gurney, A., Henzel, W., Nelson, C., Lowman, H. B., Wright, B. D., Skelton, N. J., Frantz, G. D., Tumas, D. B., Peale, F. V., Jr., Shelton, D. L., Hebert, C. C.FIZZ1, a novel cysteine-rich secreted protein associated with pulmonary inflammation, defines a new gene family.EMBO J. 19: 4046-4055, 2000. 2. Steppan, C. M., Bailey, S. T., Bhat, S., Brown, E. J., Banerjee, R. R., Wright, C. M., Patel, H. R., Ahima, R. S., Lazar, M. A.The hormone resistin links obesity to diabetes.Nature 409: 307-312, 2001. 3. Wang, H., Chu, W. S., Hemphill, C., Elbein, S. C.Human resistin gene: molecular scanning and evaluation of association with insulin sensitivity and type 2 diabetes in Caucasians.J. Clin. Endocr. Metab. 87: 2520-2524, 2002 |
Storage Buffer | Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. |
Description | Rabbit IgG polyclonal antibody for Resistin(RETN) detection. Tested with WB, IHC-P in Mouse. |
Gene Symbol | Retn |
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Gene Full Name | resistin |
Alias Symbols | AD;adipose tissue-specific secretory factor;adipose-specific cysteine-rich secreted protein A12-alpha;ADSF;cysteine-rich secreted protein FIZZ3;dominant inhibitory adipocyte-specific secretory factor;Fizz;Fizz3;found in inflammatory zone 3;r;resistin;Rstn;Xcp;Xcp4. |
NCBI Gene Id | 57264 |
Protein Name | Resistin |
Description of Target | Hormone that seems to suppress insulin ability to stimulate glucose uptake into adipose cells. Potentially links obesity to diabetes. |
Uniprot ID | Q99P87 |
Molecular Weight | 12492 MW |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "Resistin Polyclonal Antibody (OABB00427)"?
The tested species reactivity for this item is "Mouse". This antibody is predicted to have homology to "Mouse".
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How long will it take to receive "Resistin Polyclonal Antibody (OABB00427)"?
This item is available "Domestic: within 1-2 week delivery | International: within 1-2 week delivery".
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What buffer format is "Resistin Polyclonal Antibody (OABB00427)" provided in?
This item is provided in "Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "Resistin Polyclonal Antibody (OABB00427)"?
This target may also be called "AD;adipose tissue-specific secretory factor;adipose-specific cysteine-rich secreted protein A12-alpha;ADSF;cysteine-rich secreted protein FIZZ3;dominant inhibitory adipocyte-specific secretory factor;Fizz;Fizz3;found in inflammatory zone 3;r;resistin;Rstn;Xcp;Xcp4." in publications.
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What is the shipping cost for "Resistin Polyclonal Antibody (OABB00427)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "Resistin Polyclonal Antibody (OABB00427)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "Resistin Polyclonal Antibody (OABB00427)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "12492 MW".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "Resistin Polyclonal Antibody (OABB00427)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "Retn"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "Retn"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "Retn"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "Retn"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "Retn"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "Retn"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.