GNAT3 Antibody (OAEB00418)

Catalog No: OAEB00418 (Formerly GWB-EV0148)

Size:100UG

OAEB00418 (3.8ug/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Info

• Tested Species Reactivity: Human
• Predicted Species Reactivity: Dog|Human|Mouse|Rat
• Product Format: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Clonality: Polyclonal
• Host: Goat
• Application: P-ELISA, IHC, WB
• Reconstitution and Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Immunogen: GNAT3
• Purification: Ammonium Sulfate Precipitated; Affinity Purified
• Peptide Sequence: C-KNQFLDLNLKKEDKE
• Concentration: 0.5 mg/ml
• Application Info: P-ELISA: Antibody detection limit dilution 1:32000
  WB: Preliminary experiments gave an approx 27kDa band in Human Colon and Testis lysates and in cell lines NIH 3T3, A431 and Kelly after 0.1 ug/ml antibody staining. Please note that no explanation can be currently found in literature, for the band observed was given the calculated size of 40.4kDa according to NP_001095856.1. The 27kDa band was successfully blocked by incubation with the immunizing peptide.
  IHC: In paraffin embedded Human Small Intestine shows staining of endocrine cells amongst the enterocytes. Recommended concentration, 3-6 ug/ml.
• Publications:

  5-HT3A -driven green fluorescent protein delineates gustatory fibers innervating sour-responsive taste cells: A labeled line for sour taste?. J Comp Neurol. 525, 2358-2375 (2017). 28316078

  A Subset of Olfactory Sensory Neurons Express Forkhead Box J1-Driven eGFP. Chem Senses. 44, 663-671 (2019). 31504289

  Chemical receptors of the arytenoid: A comparison of human and mouse. Laryngoscope. 130, 423-430 (2020). 30908677

  Expression of Bitter Taste Receptors and Solitary Chemosensory Cell Markers in the Human Sinonasal Cavity. Chem Senses. 44, 483-495 (2019). 31231752

  Immunohistochemical Analysis of Human Vallate Taste Buds. Chem Senses. 40, 655-60 (2015). 26400924

  Michael A Kohanski, et al. Solitary chemosensory cells are a primary epithelial source of IL-25 in patients with chronic rhinosinusitis with nasal polyps. 460-469.e7 (2018). 29778504

  The Role of 5-HT3 Receptors in Signaling from Taste Buds to Nerves. J Neurosci. 35, 15984-95 (2015). 26631478

  Whole-Brain Mapping of the Expression Pattern of T1R2, a Subunit Specific to the Sweet Taste Receptor. Front Neuroanat. 15, 751839 (2021). 34776881

  Whole-Brain Mapping of the Expression Pattern of T1R2, a Subunit Specific to the Sweet Taste Receptor. Front Neuroanat. 15, 751839 (2021). 34578940

  Zika virus infection in chemosensory cells. J Neurovirol. 26, 371-381 (2020) 32144727

• Description:
  View product citations for GNAT3 Antibody (OAEB00418) on CiteAb

Target Info

• Gene Symbol: Gnat3|GNAT3
• Gene Full Name: G protein subunit alpha transducin 3|guanine nucleotide binding protein, alpha transducing 3
• Alias Symbols: alpha-gustducin;GDCA;Ggus;Ggust;gnat-3;Gt;Gtn;guanine nucleotide binding protein, alpha transducing 3;guanine nucleotide-binding protein G(t) subunit alpha-3;gustatory G protein;gustducin alpha-3 chain;gustducin, alpha polypeptide.
• NCBI Gene Id: 242851|286924|346562
• Protein Name: Guanine nucleotide-binding protein G(t) subunit alpha-3
• Uniprot ID: A8MTJ3
• Protein Accession #: NP_001095856.1
• Molecular Weight: 40.4