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U2AF1 Antibody (OAAB01511)

80% of 100
Catalog#: OAAB01511
Domestic: within 1 week delivery | International: 1 week
More Information
Predicted Species ReactivityHuman
Product FormatLiquid. PBS with 0.09% (W/V) sodium azide.
IsotypeRabbit Ig
ApplicationWB, FC, IHC-P
Additional InformationFunction: Plays a critical role in both constitutive and enhancer- dependent splicing by mediating protein-protein interactions and protein-RNA interactions required for accurate 3'-splice site selection. Recruits U2 snRNP to the branch point. Directly mediates interactions between U2AF2 and proteins bound to the enhancers and thus may function as a bridge between U2AF2 and the enhancer complex to recruit it to the adjacent intron.
Cellular Location: Nucleus. Nucleus speckle.
Reconstitution and StorageMaintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
Replacement ItemThis antibody may replace item sc-19663 from Santa Cruz Biotechnology.
ImmunogenThis U2AF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 99-125 amino acids from the Central region of human U2AF1.
PurificationThis antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Predicted Homology Based on Immunogen SequenceCow; Mouse
Datasheets/ManualsPrintable datasheet for anti-U2AF1 antibody - OAAB01511
Intended UseFor research use only and not for use in diagnostic or therapeutic procedures.
Application InfoWB: 1:1000
IHC-P: 1:50~100
FC: 1:10~50
Other Applications Image 1 DataU2AF1 Antibody (Center Y114) flow cytometry analysis of Ramos cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Gene SymbolU2AF1
Alias SymbolsSplicing factor U2AF 35 kDa subunit, U2 auxiliary factor 35 kDa subunit, U2 snRNP auxiliary factor small subunit, U2AF1, U2AF35, U2AFBP
NCBI Gene Id7307
Protein NameSplicing factor U2AF 35 kDa subunit
Description of TargetU2AF1 belongs to the splicing factor SR family. U2 auxiliary factor, comprising a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. This gene encodes the small subunit which plays a critical role in both constitutive and enhancer-dependent RNA splicing by directly mediating interactions between the large subunit and proteins bound to the enhancers.
Swissprot IdQ01081
Protein Accession #NP_001020374.1
Molecular Weight27872 Da (35 kDa subunit) Da
Tissue ToolFind tissues and cell lines supported by DNA array analysis to express U2AF1.
RNA SeqFind tissues and cell lines supported by RNA-seq analysis to express U2AF1.
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