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U2AF1 Antibody (OAAB01511)

80% of 100
Catalog#: OAAB01511
Domestic: within 1 week delivery International: 1 week
More Information
Predicted Species Reactivity Human
Product Format Liquid. PBS with 0.09% (W/V) sodium azide.
Clonality Polyclonal
Isotype Rabbit Ig
Host Rabbit
Application WB, FC, IHC-P
Additional Information Function: Plays a critical role in both constitutive and enhancer- dependent splicing by mediating protein-protein interactions and protein-RNA interactions required for accurate 3'-splice site selection. Recruits U2 snRNP to the branch point. Directly mediates interactions between U2AF2 and proteins bound to the enhancers and thus may function as a bridge between U2AF2 and the enhancer complex to recruit it to the adjacent intron.
Cellular Location: Nucleus. Nucleus speckle.
Reconstitution and Storage Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
Replacement Item This antibody may replace item sc-19663 from Santa Cruz Biotechnology.
Immunogen This U2AF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 99-125 amino acids from the Central region of human U2AF1.
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Predicted Homology Based on Immunogen Sequence Cow; Mouse
Datasheets/Manuals Printable datasheet for anti-U2AF1 antibody - OAAB01511
Intended Use For research use only and not for use in diagnostic or therapeutic procedures.
Application Info WB: 1:1000
IHC-P: 1:50~100
FC: 1:10~50
Other Applications Image 1 Data U2AF1 Antibody (Center Y114) flow cytometry analysis of Ramos cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Gene Symbol U2AF1
Alias Symbols Splicing factor U2AF 35 kDa subunit, U2 auxiliary factor 35 kDa subunit, U2 snRNP auxiliary factor small subunit, U2AF1, U2AF35, U2AFBP
NCBI Gene Id 7307
Protein Name Splicing factor U2AF 35 kDa subunit
Description of Target U2AF1 belongs to the splicing factor SR family. U2 auxiliary factor, comprising a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. This gene encodes the small subunit which plays a critical role in both constitutive and enhancer-dependent RNA splicing by directly mediating interactions between the large subunit and proteins bound to the enhancers.
Swissprot Id Q01081
Protein Accession # NP_001020374.1
Molecular Weight 27872 Da (35 kDa subunit) Da
Tissue Tool Find tissues and cell lines supported by DNA array analysis to express U2AF1.
RNA Seq Find tissues and cell lines supported by RNA-seq analysis to express U2AF1.
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