U2AF1 Antibody (OAAB01511)
|Predicted Species Reactivity||Human|
|Product Format||Liquid. PBS with 0.09% (W/V) sodium azide.|
|Application||WB, FC, IHC-P|
|Additional Information||Function: Plays a critical role in both constitutive and enhancer- dependent splicing by mediating protein-protein interactions and protein-RNA interactions required for accurate 3'-splice site selection. Recruits U2 snRNP to the branch point. Directly mediates interactions between U2AF2 and proteins bound to the enhancers and thus may function as a bridge between U2AF2 and the enhancer complex to recruit it to the adjacent intron.
Cellular Location: Nucleus. Nucleus speckle.
|Reconstitution and Storage||Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.|
|Replacement Item||This antibody may replace item sc-19663 from Santa Cruz Biotechnology.|
|Immunogen||This U2AF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 99-125 amino acids from the Central region of human U2AF1.|
|Purification||This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Predicted Homology Based on Immunogen Sequence||Cow; Mouse|
|Datasheets/Manuals||Printable datasheet for anti-U2AF1 antibody - OAAB01511|
|Intended Use||For research use only and not for use in diagnostic or therapeutic procedures.|
|Application Info||WB: 1:1000
|Other Applications Image 1 Data||U2AF1 Antibody (Center Y114) flow cytometry analysis of Ramos cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.|
|Alias Symbols||Splicing factor U2AF 35 kDa subunit, U2 auxiliary factor 35 kDa subunit, U2 snRNP auxiliary factor small subunit, U2AF1, U2AF35, U2AFBP|
|NCBI Gene Id||7307|
|Protein Name||Splicing factor U2AF 35 kDa subunit|
|Description of Target||U2AF1 belongs to the splicing factor SR family. U2 auxiliary factor, comprising a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. This gene encodes the small subunit which plays a critical role in both constitutive and enhancer-dependent RNA splicing by directly mediating interactions between the large subunit and proteins bound to the enhancers.
|Protein Accession #||NP_001020374.1|
|Molecular Weight||27872 Da (35 kDa subunit) Da|
|Tissue Tool||Find tissues and cell lines supported by DNA array analysis to express U2AF1.|
|RNA Seq||Find tissues and cell lines supported by RNA-seq analysis to express U2AF1.|
Write Your Own Review