Size:50ug
$199.00
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AMM99021
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5-Methylcytosine Antibody (AMM99021)

Rating:
100% of 100
Catalog#: AMM99021
Domestic: within 24 hours delivery International: 3-5 business days
More Information
Predicted Species Reactivity Human, Zebrafish, Dog, Mouse, Rat, Pig, Chicken
Product Format Liquid.PBS pH 7.4 (NaCl 137 mM, KCI 2.7 mM, 10 mM Na2HPO4, 2 mM KH2PO4). No preservative.
Clonality Monoclonal
Clone 33D3
Isotype IgG1
Host Mouse
Application FC, IHC-FP, ICC, IP, CHIP, RIA, IF
Additional Information Sterility Test: 0 CFU (on blood agar).
Reconstitution and Storage Ready for usage. For longer periods of storage, store at -20C. Can be stored 1 month at 4C.Avoid repeat freeze-thaw cycles.
Purification Protein A ceramic hyper DF affinity chromatography
Concentration 1 mg/ml
Datasheets/Manuals Printable datasheet for AMM99021
Purity > 95% (Gel electrophoresis SDS PAGE. Densitometry analysis)
Intended Use For research use only.
Publications

Fukuda, K. et al. Regional DNA methylation differences between humans and chimpanzees are associated with genetic changes, transcriptional divergence and disease genes. J. Hum. Genet. 58, 446-54 (2013). ELISA, FC, IHC-FP, ICC, IP, CHIP, RIA, IF, Human, Zebrafish, Dog, Mouse, Rat, Pig, Chicken23739127

Gertych, A., Oh, J. H., Wawrowsky, K. A., Weisenberger, D. J. & Tajbakhsh, J. 3-D DNA methylation phenotypes correlate with cytotoxicity levels in prostate and liver cancer cell models. BMC Pharmacol. Toxicol. 14, 11 (2013). ELISA, FC, IHC-FP, ICC, IP, CHIP, RIA, IF, Human, Zebrafish, Dog, Mouse, Rat, Pig, Chicken23394161

Jensen, T. J., Novak, P., Eblin, K. E., Gandolfi, A. J. & Futscher, B. W. Epigenetic remodeling during arsenical-induced malignant transformation. Carcinogenesis 29, 1500-8 (2008). ELISA, FC, IHC-FP, ICC, IP, CHIP, RIA, IF, Human, Zebrafish, Dog, Mouse, Rat, Pig, Chicken18448484

Kurita, R. & Niwa, O. DNA methylation analysis triggered by bulge specific immuno-recognition. Anal. Chem. 84, 7533-8 (2012). ELISA, FC, IHC-FP, ICC, IP, CHIP, RIA, IF, Human, Zebrafish, Dog, Mouse, Rat, Pig, Chicken22880797

Kurita, R., Arai, K., Nakamoto, K., Kato, D. & Niwa, O. Determination of DNA methylation using electrochemiluminescence with surface accumulable coreactant. Anal. Chem. 84, 1799-803 (2012). ELISA, FC, IHC-FP, ICC, IP, CHIP, RIA, IF, Human, Zebrafish, Dog, Mouse, Rat, Pig, Chicken22263690

Macaulay, E. C., Weeks, R. J., Andrews, S. & Morison, I. M. Hypomethylation of functional retrotransposon-derived genes in the human placenta. Mamm. Genome 22, 722-35 (2011). ELISA, FC, IHC-FP, ICC, IP, CHIP, RIA, IF, Human, Zebrafish, Dog, Mouse, Rat, Pig, Chicken21874386

Maki, W. C. et al. Nanowire-transistor based ultra-sensitive DNA methylation detection. Biosens. Bioelectron. 23, 780-7 (2008). ELISA, FC, IHC-FP, ICC, IP, CHIP, RIA, IF, Human, Zebrafish, Dog, Mouse, Rat, Pig, Chicken17936611

Minardi, D. et al. Prognostic role of global DNA-methylation and histone acetylation in pT1a clear cell renal carcinoma in partial nephrectomy specimens. J. Cell. Mol. Med. 13, 2115-21 (2009). ELISA, FC, IHC-FP, ICC, IP, CHIP, RIA, IF, Human, Zebrafish, Dog, Mouse, Rat, Pig, Chicken18752633

Ribas, R. et al. Members of the TEAD family of transcription factors regulate the expression of Myf5 in ventral somitic compartments. Dev. Biol. 355, 372-80 (2011). ELISA, FC, IHC-FP, ICC, IP, CHIP, RIA, IF, Human, Zebrafish, Dog, Mouse, Rat, Pig, Chicken21497756

Tajbakhsh, J. Covisualization of methylcytosine, global DNA, and protein biomarkers for In Situ 3D DNA methylation phenotyping of stem cells. Methods Mol. Biol. 1052, 77-88 (2013). ELISA, FC, IHC-FP, ICC, IP, CHIP, RIA, IF, Human, Zebrafish, Dog, Mouse, Rat, Pig, Chicken23592032

Vrba, L., Junk, D. J., Novak, P. & Futscher, B. W. p53 induces distinct epigenetic states at its direct target promoters. BMC Genomics 9, 486 (2008). ELISA, FC, IHC-FP, ICC, IP, CHIP, RIA, IF, Human, Zebrafish, Dog, Mouse, Rat, Pig, Chicken18922183

Walsh, T. K. et al. A functional DNA methylation system in the pea aphid, Acyrthosiphon pisum. Insect Mol. Biol. 19 Suppl 2, 215-28 (2010). ELISA, FC, IHC-FP, ICC, IP, CHIP, RIA, IF, Human, Zebrafish, Dog, Mouse, Rat, Pig, Chicken20482652

Zhang, W., Lee, H.-R., Koo, D.-H. & Jiang, J. Epigenetic modification of centromeric chromatin: hypomethylation of DNA sequences in the CENH3-associated chromatin in Arabidopsis thaliana and maize. Plant Cell 20, 25-34 (2008). ELISA, FC, IHC-FP, ICC, IP, CHIP, RIA, IF, Human, Zebrafish, Dog, Mouse, Rat, Pig, Chicken18239133

Zhang, W., Wang, X., Yu, Q., Ming, R. & Jiang, J. DNA methylation and heterochromatinization in the male-specific region of the primitive Y chromosome of papaya. Genome Res. 18, 1938-43 (2008). ELISA, FC, IHC-FP, ICC, IP, CHIP, RIA, IF, Human, Zebrafish, Dog, Mouse, Rat, Pig, Chicken18593814

Target Reference Maki W. C., et al., Biosens Bioelectron 23(6):780-787 (2008).
Walsh, T. K., et al., Insect Mol Biol. 19 Suppl 2:215-28 (2010).
Brena, R. M., et al., Methods in Molecular Biology, 507, Part IV, 257-269 (2009).
Zhang, W., et al., Plant Cell. 20(1):25-34 (2008).
Zhang, W., et al, Genome Res. 18(12):1938-1943 (2008).
Jensen, T. J., et al., Carcinogenesis.29(8):1500-1508 (2008).
Maki W. C., et al., Biosens Bioelectron 23(6):780-787 (2008).
Vrba, L., et al., BMC Genomics. 15;9:486 (2008).
Vinsky, M. D., et al., Reprod Fertil Dev. 19(5):603-611 (2007).
Novak, P., et al., Cancer Res. 66(22):10664-10670 (2006).
Description of Target Methylation of DNA cytosine bases plays important roles in the regulation of gene transcription. It has been estimated that 60 to 90% of the cytosines in CpG dinucleotides are methylated. An increase in methyl-CpG correlates with transcriptional silencing for the whole chromosome especially in developmentally regulated genes. Methylation is believed to lead to transcriptional silencing by multiple mechanisms including alteration of transcription factor binding to promoters and alteration in chromatin structure.
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