Size:50ug
$199.00
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SKU
AMM99021
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5-Methylcytosine Antibody (AMM99021)

Rating:
100% of 100
Catalog#: AMM99021
Domestic: within 24 hours delivery | International: 3-5 days
More Information
Predicted Species Reactivity Human, Zebrafish, Dog, Mouse, Rat, Pig, Chicken
Product Format Liquid.PBS pH 7.4 (NaCl 137 mM, KCI 2.7 mM, 10 mM Na2HPO4, 2 mM KH2PO4). No preservative.
Clonality Monoclonal
Clone 33D3
Isotype IgG1
Host Mouse
Application FC, IHC-FP, ICC, IP, CHIP, RIA, IF
Additional Information Sterility Test: 0 CFU (on blood agar).
Reconstitution and Storage Ready for usage. For longer periods of storage, store at -20C. Can be stored 1 month at 4C.Avoid repeat freeze-thaw cycles.
Purification Protein A ceramic hyper DF affinity chromatography
Concentration 1 mg/ml
Datasheets/Manuals Printable datasheet for AMM99021
Purity > 95% (Gel electrophoresis SDS PAGE. Densitometry analysis)
Intended Use For research use only.
Publications

Fukuda, K. et al. Regional DNA methylation differences between humans and chimpanzees are associated with genetic changes, transcriptional divergence and disease genes. J. Hum. Genet. 58, 446-54 (2013). ELISA, FC, IHC-FP, ICC, IP, CHIP, RIA, IF, Human, Zebrafish, Dog, Mouse, Rat, Pig, Chicken23739127

Gertych, A., Oh, J. H., Wawrowsky, K. A., Weisenberger, D. J. & Tajbakhsh, J. 3-D DNA methylation phenotypes correlate with cytotoxicity levels in prostate and liver cancer cell models. BMC Pharmacol. Toxicol. 14, 11 (2013). ELISA, FC, IHC-FP, ICC, IP, CHIP, RIA, IF, Human, Zebrafish, Dog, Mouse, Rat, Pig, Chicken23394161

Jensen, T. J., Novak, P., Eblin, K. E., Gandolfi, A. J. & Futscher, B. W. Epigenetic remodeling during arsenical-induced malignant transformation. Carcinogenesis 29, 1500-8 (2008). ELISA, FC, IHC-FP, ICC, IP, CHIP, RIA, IF, Human, Zebrafish, Dog, Mouse, Rat, Pig, Chicken18448484

Kurita, R. & Niwa, O. DNA methylation analysis triggered by bulge specific immuno-recognition. Anal. Chem. 84, 7533-8 (2012). ELISA, FC, IHC-FP, ICC, IP, CHIP, RIA, IF, Human, Zebrafish, Dog, Mouse, Rat, Pig, Chicken22880797

Kurita, R., Arai, K., Nakamoto, K., Kato, D. & Niwa, O. Determination of DNA methylation using electrochemiluminescence with surface accumulable coreactant. Anal. Chem. 84, 1799-803 (2012). ELISA, FC, IHC-FP, ICC, IP, CHIP, RIA, IF, Human, Zebrafish, Dog, Mouse, Rat, Pig, Chicken22263690

Macaulay, E. C., Weeks, R. J., Andrews, S. & Morison, I. M. Hypomethylation of functional retrotransposon-derived genes in the human placenta. Mamm. Genome 22, 722-35 (2011). ELISA, FC, IHC-FP, ICC, IP, CHIP, RIA, IF, Human, Zebrafish, Dog, Mouse, Rat, Pig, Chicken21874386

Maki, W. C. et al. Nanowire-transistor based ultra-sensitive DNA methylation detection. Biosens. Bioelectron. 23, 780-7 (2008). ELISA, FC, IHC-FP, ICC, IP, CHIP, RIA, IF, Human, Zebrafish, Dog, Mouse, Rat, Pig, Chicken17936611

Minardi, D. et al. Prognostic role of global DNA-methylation and histone acetylation in pT1a clear cell renal carcinoma in partial nephrectomy specimens. J. Cell. Mol. Med. 13, 2115-21 (2009). ELISA, FC, IHC-FP, ICC, IP, CHIP, RIA, IF, Human, Zebrafish, Dog, Mouse, Rat, Pig, Chicken18752633

Ribas, R. et al. Members of the TEAD family of transcription factors regulate the expression of Myf5 in ventral somitic compartments. Dev. Biol. 355, 372-80 (2011). ELISA, FC, IHC-FP, ICC, IP, CHIP, RIA, IF, Human, Zebrafish, Dog, Mouse, Rat, Pig, Chicken21497756

Tajbakhsh, J. Covisualization of methylcytosine, global DNA, and protein biomarkers for In Situ 3D DNA methylation phenotyping of stem cells. Methods Mol. Biol. 1052, 77-88 (2013). ELISA, FC, IHC-FP, ICC, IP, CHIP, RIA, IF, Human, Zebrafish, Dog, Mouse, Rat, Pig, Chicken23592032

Vrba, L., Junk, D. J., Novak, P. & Futscher, B. W. p53 induces distinct epigenetic states at its direct target promoters. BMC Genomics 9, 486 (2008). ELISA, FC, IHC-FP, ICC, IP, CHIP, RIA, IF, Human, Zebrafish, Dog, Mouse, Rat, Pig, Chicken18922183

Walsh, T. K. et al. A functional DNA methylation system in the pea aphid, Acyrthosiphon pisum. Insect Mol. Biol. 19 Suppl 2, 215-28 (2010). ELISA, FC, IHC-FP, ICC, IP, CHIP, RIA, IF, Human, Zebrafish, Dog, Mouse, Rat, Pig, Chicken20482652

Zhang, W., Lee, H.-R., Koo, D.-H. & Jiang, J. Epigenetic modification of centromeric chromatin: hypomethylation of DNA sequences in the CENH3-associated chromatin in Arabidopsis thaliana and maize. Plant Cell 20, 25-34 (2008). ELISA, FC, IHC-FP, ICC, IP, CHIP, RIA, IF, Human, Zebrafish, Dog, Mouse, Rat, Pig, Chicken18239133

Zhang, W., Wang, X., Yu, Q., Ming, R. & Jiang, J. DNA methylation and heterochromatinization in the male-specific region of the primitive Y chromosome of papaya. Genome Res. 18, 1938-43 (2008). ELISA, FC, IHC-FP, ICC, IP, CHIP, RIA, IF, Human, Zebrafish, Dog, Mouse, Rat, Pig, Chicken18593814

Target Reference Maki W. C., et al., Biosens Bioelectron 23(6):780-787 (2008).
Walsh, T. K., et al., Insect Mol Biol. 19 Suppl 2:215-28 (2010).
Brena, R. M., et al., Methods in Molecular Biology, 507, Part IV, 257-269 (2009).
Zhang, W., et al., Plant Cell. 20(1):25-34 (2008).
Zhang, W., et al, Genome Res. 18(12):1938-1943 (2008).
Jensen, T. J., et al., Carcinogenesis.29(8):1500-1508 (2008).
Maki W. C., et al., Biosens Bioelectron 23(6):780-787 (2008).
Vrba, L., et al., BMC Genomics. 15;9:486 (2008).
Vinsky, M. D., et al., Reprod Fertil Dev. 19(5):603-611 (2007).
Novak, P., et al., Cancer Res. 66(22):10664-10670 (2006).
Description of Target Methylation of DNA cytosine bases plays important roles in the regulation of gene transcription. It has been estimated that 60 to 90% of the cytosines in CpG dinucleotides are methylated. An increase in methyl-CpG correlates with transcriptional silencing for the whole chromosome especially in developmentally regulated genes. Methylation is believed to lead to transcriptional silencing by multiple mechanisms including alteration of transcription factor binding to promoters and alteration in chromatin structure.
  1. What is the species homology for "5-Methylcytosine Antibody (AMM99021)"?

    The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human, Zebrafish, Dog, Mouse, Rat, Pig, Chicken".

  2. How long will it take to receive "5-Methylcytosine Antibody (AMM99021)"?

    This item is available "Domestic: within 24 hours delivery | International: 3-5 days".

  3. What buffer format is "5-Methylcytosine Antibody (AMM99021)" provided in?

    This item is provided in "Liquid.PBS pH 7.4 (NaCl 137 mM, KCI 2.7 mM, 10 mM Na2HPO4, 2 mM KH2PO4). No preservative.".
    Additional format options may be available. For more information please contact info@avivasysbio.com.

  4. What are other names for "5-Methylcytosine Antibody (AMM99021)"?

    This target may also be called "" in publications.

  5. What is the shipping cost for "5-Methylcytosine Antibody (AMM99021)"?

    The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.

  6. What is the guarantee for "5-Methylcytosine Antibody (AMM99021)"?

    All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.

  7. Can I get bulk pricing for "5-Methylcytosine Antibody (AMM99021)"?

    You can get bulk pricing for this item by going here.

  8. What is the molecular weight of the protein?

    The molecular weight reported by Uniprot for this item is "".
    Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics.

  9. What protocols are available for "5-Methylcytosine Antibody (AMM99021)"?

    We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data tab on the product page.

  10. What are positive controls for ""?

    We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.

  11. What are negative controls for ""?

    We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.

  12. What other proteins interact with ""?

    This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.

  13. What biological processes are associated with ""?

    This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.

  14. What cellular components are associated with ""?

    This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.

  15. What protein functions are associated with ""?

    This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.

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