- Gene Symbol:
- NCBI Gene Id:
- Official Gene Full Name:
- SRY (sex determining region Y)-box 10
- Protein Name:
- Transcription factor SOX-10
- Swissprot Id:
- Protein Accession #:
- Nucleotide Accession #:
- Alias Symbols:
- DOM, MGC15649, WS2E, WS4, PCWH, WS4C
- Replacement Item:
- This antibody may replace item sc-17342 from Santa Cruz Biotechnology.
- Description of Target:
- SOX10 is a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. This protein may act as a transcriptional activator after forming a protein complex with other proteins. It acts as a nucleocytoplasmic shuttle protein and is important for neural crest and peripheral nervous system development. Mutations in this gene are associated with Waardenburg-Shah and Waardenburg-Hirschsprung disease.
- Protein Size (# AA):
- Molecular Weight:
- Affinity Purified
- IHC, WB
- Tissue Tool:
- Find tissues and cell lines supported by DNA array analysis to express SOX10.
- RNA Seq:
- Find tissues and cell lines supported by RNA-seq analysis to express SOX10.
- The immunogen is a synthetic peptide directed towards the middle region of human SOX10
- Species Reactivity:
- Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat
- Predicted Homology Based on Immunogen Sequence:
- Cow: 93%; Dog: 93%; Guinea Pig: 93%; Horse: 93%; Human: 100%; Mouse: 93%; Rabbit: 93%; Rat: 86%
- Complete computational species homology data:
- Anti-SOX10 (ARP33326_P050)
- Peptide Sequence:
- Synthetic peptide located within the following region: PGGEAEQGGTAAIQAHYKSAHLDHRHPGEGSPMSDGNPEHPSGQSHGPPT
- Product Format:
- Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
- Reconstitution and Storage:
- For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
- Batch dependent within range: 100 ul at 0.5 - 1 mg/ml
- Protein Interactions:
- UBC; CDK6; EXOC5; NMI; EPAS1; POU3F2; UTF1; PAX3; UBE2I; OLIG2; HHEX; PAX6; ALX4; PRRX2; SUMO2; POU3F3; SUMO3; SOX10; SUMO1; TCF12; MEOX1; DLX5; HIVEP1; SP1; PRRX1; JUN; POU5F1; HOXC4; HOXA3; EGR2; CEBPA;
- Blocking Peptide:
- For anti-SOX10 (ARP33326_P050) antibody is Catalog # AAP33326
- Printable datasheet for anti-SOX10 (ARP33326_P050) antibody
Wilson, NR; Olm-Shipman, AJ; Acevedo, DS; Palaniyandi, K; Hall, EG; Kosa, E; Stumpff, KM; Smith, GJ; Pitstick, L; Liao, EC; Bjork, BC; Czirok, A; Saadi, I; SPECC1L deficiency results in increased adherens junction stability and reduced cranial neural crest cell delamination. 6, 17735 (2016). IHC, WB, Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat 26787558
What type of experimental sample are you using and how did you prepare it?
We are using HEK293 whole cell lysates with Aviva protocol.
What applications did you test the antibody in? Please include dilutions of the primary and secondary reagents.
The primary antibodies were tested in Western blot experiments at 1:4,000, secondary antibody was used at 1:10,000 dilution.
What controls were used in your experiment? Please include your positive control.
p53 is used as a positive control; protein transfer was monitored via Ponceau staining.
How did you store the antibody after re-suspension?
Frozen at -20 C in aliquots.
Please provide the protocol for your application procedure. Please be as detailed as possible.
Experiment done using the Aviva protocol. All antibodies show virtually no background.
How would you rate this antibody on a scale from 1-5? Why?
excellent (5, produced unique bands with expected sizes)
Would you use this antibody in future experiments?