Renin-1 ELISA Kit (Mouse) (OKBB00560)

Catalog No: OKBB00560

Size:96 Wells

Mouse Renin-1 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Mouse
• Application: ELISA
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: 2.5 Hours
• ELISA Kit Principle: Aviva's mouse Renin-1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A polyclonal antibody from goat specific for Renin-1 has been precoated onto 96-well plates. Standards(NS0, S72-R402) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Renin-1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the mouse Renin-1 amount of sample captured in plate.
• ELISA Kit Range: 62.5 pg/ml - 4,000 pg/ml
• ELISA Kit Reproducibility:

  	Intra-Assay Precision			Inter-Assay Precision
  Sample	1	2	3	1	2	3
  n	16	16	16	24	24	24
  Mean (pg/mL)	890	1137	2932	927	2274	3045
  SD	49.84	48.9	161.26	67.7	129.6	194.88
  CV%	5.6	4.3	5.5	7.3	5.7	6.4

• ELISA Kit Component:
  • 96-Well plate Pre-coated with Anti-Mouse Renin-1 Antibody: 1 x (12 x 8 Well Strip)
  • Lyophilized Recombinant Mouse Renin-1 Standard: 2 x 10 ng/tube
  • 100X Biotinylated Anti-Mouse Renin-1 Antibody: 1 x 130 uL
  • 100X Avidin-Biotin-Peroxidase Complex (ABC): 1 x 130 uL
  • Sample Diluent Buffer: 1 x 30 mL
  • Antibody Diluent Buffer: 1 x 12 mL
  • ABC Diluent Buffer: 1 x 12 mL
  • TMB Color Developing Agent: 1 x 10 mL
  • TMB Stop Solution: 1 x 10 mL
  • 10X Wash Buffer: 1 x 30 mL
• Reconstitution and Storage: Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
• Sample Type: Cell culture supernatants, serum and plasma (heparin, EDTA)
• Sensitivity: <10 pg/ml
• Specificity: Natural and recombinant mouse Renin-1
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay
• Immunogen: Expression system for standard: NS0; Immunogen sequence: S72-R402
• Description: For quantitative detection of mouse Renin-1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Target Info

• Gene Symbol: REN1
• Gene Full Name: renin 1 structural
• Alias Symbols: ATP6AP2, ATP6M8-9, ATPase H(+)-transporting lysosomal accessory protein 2, ATPase H(+)-transporting lysosomal-interacting protein 2, Embryonic liver differentiation factor 10, ER-localized type I transmembrane adaptor, N14F, Renin receptor, Renin/prorenin receptor, RENR_HUMAN, V-ATPase M8.9 subunit, Vacuolar ATP synthase membrane sector-associated protein M8-9
• NCBI Gene Id: 19701
• Protein Name: Renin-1
• Description of Target: Renin-1 is also known as Ren, Angiotensinogenase or Kidney rennin. It is a member of peptidase A1 family. Renin is a highly specific endopeptidase, whose only known function is to generate angiotensin I from angiotensinogen in the plasma, initiating a cascade of reactions that produce an elevation of blood pressure and increased sodium retention by the kidney. It associated to membranes via binding to ATP6AP2.
• Uniprot ID: P06281
• Protein Accession #: NP_112469.1
• Nucleotide Accession #: NM_031192.3