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Catalog No: OKBB00553
Size:96 Wells
Price: $583.00
SKU
OKBB00553
Availability: Domestic: within 1-2 week delivery | International: within 1-2 week delivery
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityMouse
ApplicationELISA
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration2.5 Hours
ELISA Kit PrincipleAviva's mouse Relaxin 1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for Relaxin 1 has been precoated onto 96-well plates. Standards(E.coli, R23-R54(B) & E161-C185(A)) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Relaxin 1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the mouse Relaxin 1 amount of sample captured in plate.
ELISA Kit Range78 pg/ml - 5,000 pg/ml
ELISA Kit Reproducibility
Intra-Assay PrecisionInter-Assay Precision
Sample123123
n 161616242424
Mean (pg/mL)6881402236665115832425
SD45.4160.313048.295174.6
CV%6.64.35.57.467.2
ELISA Kit Component
ComponentAmount
96-Well plate Pre-coated with Anti-Mouse Relaxin 1 Antibody1 x (12 x 8 Well Strip)
Lyophilized Recombinant Mouse Relaxin 1 Standard2 x 10 ng/tube
100X Biotinylated Anti-Mouse Relaxin 1 Antibody1 x 130 uL
100X Avidin-Biotin-Peroxidase Complex (ABC)1 x 130 uL
Sample Diluent Buffer1 x 30 mL
Antibody Diluent Buffer1 x 12 mL
ABC Diluent Buffer1 x 12 mL
TMB Color Developing Agent1 x 10 mL
TMB Stop Solution1 x 10 mL
10X Wash Buffer1 x 30 mL
Reconstitution and StorageStore at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Sample TypeCell culture supernatants, serum and plasma (heparin)
Sensitivity<10 pg/ml
SpecificityNatural and recombinant mouse Relaxin 1
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
ImmunogenExpression system for standard: E.coli; Immunogen sequence: R23-R54 (B) & E161-C185 (A)
DescriptionFor quantitative detection of mouse Relaxin 1 in cell culture supernatants, serum and plasma(heparin, EDTA).
Gene SymbolRLN1
Gene Full Namerelaxin 1
Alias SymbolsH1RLX, Preprorelaxin H1, PreprorelaxinH1, Prorelaxin, Prorelaxin H1, ProrelaxinH1, REL1_HUMAN, Relaxin 1 (H1), Relaxin A chain, Relaxin H1, Relaxin1, RelaxinH1, RLN 1, RLN1, RLX H1, RLXH 1, RLXH
NCBI Gene Id19773
Protein NameProrelaxin 1
Description of TargetRelaxin 1 is a member of relaxin-like peptide family. Relaxin gene maps to mouse chromosome 19 near D19Mit23. Relaxin is a peptide hormone produced by the corpora lutea of ovaries during pregnancy in many mammalian species, including man. Relaxin widens the pubic bone and facilitates labor, it also softens the cervix (cervical ripening), and relaxes the uterine musculature. However, its significance may reach much further. Relaxin affects collagen metabolism, inhibiting collagen synthesis and enhancing its breakdown by increasing matrix metalloproteinases. It also enhances angiogenesis and is a potent renal vasodilator.
Uniprot IDP47932
Protein Accession #NP_035402
Nucleotide Accession #NM_011272
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