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Catalog No: OKDD00611 (Formerly GWB-KBBWS9)
Size:96 Wells
Price: $775.00
SKU
OKDD00611
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Datasheets/ManualsPrintable datasheet for RELA ELISA Kit (Rat) (OKDD00611)
Product Info
Predicted Species ReactivityRat|Rattus norvegicus
ApplicationEnzyme-linked immunosorbent assay
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~4 Hours
ELISA Kit PrincipleAviva Systems Biology RELA ELISA Kit (Rat) (OKDD00611) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody for RELA has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for RELA is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample RELA captured in well.
ELISA Kit Range0.156-10ng/mL
ELISA Kit Reproducibility

Intra-assay Precision: 3 samples with low, middle and high level analyte were measured with 20 replicates on one plate, respectively.

Inter-assay Precision: 3 samples with low, middle and high level analyte were measured across 3 different plates, with 8 replicates per plate.

Mean Intra-Assay: CV<10%

Mean Inter-Assay: CV<12%

ELISA Kit Component
ComponentAmount
Anti-RELA Microplate96 Wells (12 x 8 Well Strips)
RELA Lyophilized Standard2 x 10 ng
Standard Diluent1 x 20 mL
100X RELA Biotinylated Detector Antibody1 x 120 uL
100X Avidin-HRP Detection Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Detection Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, plasma, tissue homogenates, cell lysates
Sensitivity<0.049 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of RELA. No significant cross-reactivity or interference between RELA and analogues was observed.
Assay InfoQuantitative Sandwich ELISA
Gene SymbolRela
Gene Full NameRELA proto-oncogene, NF-kB subunit
Alias SymbolsNFkB;nos2;nuclear factor kappa B subunit p65;nuclear factor kappaB subunit p65;transcription factor p65;v-rel avian reticuloendotheliosis viral oncogene homolog A;v-rel reticuloendotheliosis viral oncogene homolog A.
NCBI Gene Id309165
Protein NameRELA proto-oncogene, NF-kB subunit
Description of TargetComponent of the nf-kappa b complex; involved in transcriptional activation [rgd, feb 2006]
Uniprot IDQ7TQN4
Protein Accession #NP_954888.1
Nucleotide Accession #NM_199267.2
Protein Size (# AA)550
Molecular Weight60 kDa
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