Catalog No: OKDD00611 (Formerly GWB-KBBWS9)
Size:96 Wells
Price: $775.00
SKU
OKDD00611
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
Contact Us:
- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
Shipping Info:
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
Datasheets/Manuals | Printable datasheet for RELA ELISA Kit (Rat) (OKDD00611) |
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Predicted Species Reactivity | Rat|Rattus norvegicus | ||||||||||||||||||||||
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Application | Enzyme-linked immunosorbent assay | ||||||||||||||||||||||
ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||
ELISA Kit Duration | ~4 Hours | ||||||||||||||||||||||
ELISA Kit Principle | Aviva Systems Biology RELA ELISA Kit (Rat) (OKDD00611) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody for RELA has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for RELA is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample RELA captured in well. | ||||||||||||||||||||||
ELISA Kit Range | 0.156-10ng/mL | ||||||||||||||||||||||
ELISA Kit Reproducibility | Intra-assay Precision: 3 samples with low, middle and high level analyte were measured with 20 replicates on one plate, respectively. Inter-assay Precision: 3 samples with low, middle and high level analyte were measured across 3 different plates, with 8 replicates per plate. Mean Intra-Assay: CV<10% Mean Inter-Assay: CV<12% | ||||||||||||||||||||||
ELISA Kit Component |
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Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
Sample Type | Serum, plasma, tissue homogenates, cell lysates | ||||||||||||||||||||||
Sensitivity | <0.049 ng/mL | ||||||||||||||||||||||
Specificity | This assay has high sensitivity and excellent specificity for detection of RELA. No significant cross-reactivity or interference between RELA and analogues was observed. | ||||||||||||||||||||||
Assay Info | Quantitative Sandwich ELISA |
Gene Symbol | Rela |
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Gene Full Name | RELA proto-oncogene, NF-kB subunit |
Alias Symbols | NFkB;nos2;nuclear factor kappa B subunit p65;nuclear factor kappaB subunit p65;transcription factor p65;v-rel avian reticuloendotheliosis viral oncogene homolog A;v-rel reticuloendotheliosis viral oncogene homolog A. |
NCBI Gene Id | 309165 |
Protein Name | RELA proto-oncogene, NF-kB subunit |
Description of Target | Component of the nf-kappa b complex; involved in transcriptional activation [rgd, feb 2006] |
Uniprot ID | Q7TQN4 |
Protein Accession # | NP_954888.1 |
Nucleotide Accession # | NM_199267.2 |
Protein Size (# AA) | 550 |
Molecular Weight | 60 kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
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