RELA Antibody - FITC Conjugated (OACA01134)

Catalog No: OACA01134

Size:100ug

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Conjugation: FITC
• Application: WB
• Reconstitution and Storage: -20°C or -80°C
• Immunogen: Recombinant Human Transcription factor p65 protein (1-210AA)
• Purification: Protein G Purified
• Purity: Caprylic Acid Ammonium Sulfate Precipitation purified
• Reference: [1] Isolation of a rel-related human cDNA that potentially encodes the 65-kD subunit of NF-kappa B.'Ruben S.M., Dillon P.J., Schreck R., Henkel T., Chen C.-H., Maher M., Baeuerle P.A., Rosen C.A.Science 251:1490-1493(1991)
  [PubMed]
  [Europe PMC]
  [Abstract] Cited for: NUCLEOTIDE SEQUENCE
  [MRNA] (ISOFORM 1).
  [2] Genomic organization of the gene encoding the p65 subunit of NF-kappa B: multiple variants of the p65 protein may be generated by alternative splicing.'Deloukas P., van Loon A.P.G.M.Hum. Mol. Genet. 2:1895-1900(1993)
  [3] An alternatively spliced transcript, p65 delta 2, of the gene encoding the p65 subunit of the transcription factor NF-kappa B.'Lyle R., Valleley E.M., Sharpe P.T., Hewitt J.E.Gene 138:265-266(1994)
• Storage Buffer: Preservative: 0.03% Proclin 300Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Target Info

• Gene Symbol: RELA
• Gene Full Name: RELA proto-oncogene, NF-kB subunit
• Alias Symbols: CMCU;NF-kappa-B p65delta3;NF-kappa-B transcription factor p65;NFKB3;nuclear factor NF-kappa-B p65 subunit;nuclear factor of kappa light polypeptide gene enhancer in B-cells 3;p65;transcription factor p65;v-rel avian reticuloendotheliosis viral oncogene homolog A.
• NCBI Gene Id: 5970
• Protein Name: Transcription factor p65
• Description of Target: NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. NF-kappa-B heterodimeric p65-p50 and p65-c-Rel complexes are transcriptional activators. The NF-kappa-B p65-p65 complex appears to be involved in invasin-mediated activation of IL-8 expression. The inhibitory effect of I-kappa-B upon NF-kappa-B the cytoplasm is exerted primarily through the interaction with p65. p65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex. Associates with chromatin at the NF-kappa-B promoter region via association with DDX1. Essential for cytokine gene expression in T-cells (PubMed:15790681).
• Uniprot ID: Q04206