REL ELISA Kit (Human) (OKEH08241)

Catalog No: OKEH08241

Size:96T

Human REL ELISA Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Principle: Aviva Systems Biology REL ELISA Kit (Human) (OKEH08241) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for REL has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for REL is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample REL captured in well.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 102% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=6.4% (n = 20)
  Mean Inter-assay CV%: <=11.2% (n = 20)
• ELISA Kit Component:
  • REL Microplate: 96 Wells (12 x 8 Well strips)
  • REL Lyophilized Standard: 2
  • 100X Biotinylated REL Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Function: Proto-oncogene that may play a role in differentiation and lymphopoiesis. NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. The NF-kappa-B heterodimer RELA/p65-c-Rel is a transcriptional activator.
  Subcellular Location: Nucleus
  Unigene: Hs.631886
  SMR: Q04864
  String: 9606.ENSP00000295025
  KEGG: hsa:5966
  MIM: 164910
  Pfam: PF16179
• Reconstitution and Storage: Store as indicated in product manual.
• Sensitivity: 0.183ng/mL
• Specificity: Natural and recombinant Human Proto-oncogene c-Rel
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: REL
• Gene Full Name: REL proto-oncogene, NF-kB subunit
• Alias Symbols: C-Rel;HIVEN86A;oncogene REL, avian reticuloendotheliosis;proto-oncogene c-Rel;v-rel avian reticuloendotheliosis viral oncogene homolog.
• NCBI Gene Id: 5966
• Protein Name: Q04864
• Description of Target: Proto-oncogene c-Rel
• Uniprot ID: https://www.uniprot.org/uniprot/Q04864
• Protein Accession #: NP_001278675.1
• Nucleotide Accession #: NM_001291746.1