REG3A ELISA Kit (Rat) (OKCD08685)

Catalog No: OKCD08685

Size:96WELLS

REG3A ELISA Kit (Rat) (OKCD08685) Standard Curve

Product Info

• Predicted Species Reactivity: Rat|Rattus norvegicus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Regenerating Islet Derived Protein 3 Alpha (REG3a). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Regenerating Islet Derived Protein 3 Alpha (REG3a). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Regenerating Islet Derived Protein 3 Alpha (REG3a), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Regenerating Islet Derived Protein 3 Alpha (REG3a) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Regenerating Islet Derived Protein 3 Alpha (REG3a) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Regenerating Islet Derived Protein 3 Alpha (REG3a) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • REG3A Microplate: 96 Wells (12 x 8 Well strips)
  • REG3A Lyophilized Standard: 2
  • 100X REG3A HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: < 0.133ng/mL

Target Info

• Gene Symbol: Reg3a
• Gene Full Name: regenerating islet-derived 3 alpha
• Alias Symbols: islet of Langerhans regenerating protein 3;lithostathine 3;pancreatitis-associated protein 2;Pap2;PapII;REG 3;reg III-alpha;REG-3-alpha;regenerating islet-derived protein 3-alpha;regenerating islet-derived protein III-alpha;regIII.
• NCBI Gene Id: 171162
• Protein Name: Regenerating islet-derived protein 3-alpha
• Description of Target: Bactericidal C-type lectin. Regulates keratinocyte proliferation and differentiation after skin injury via activation of EXTL3-PI3K-AKT signaling pathway (By similarity).
• Uniprot ID: P35231
• Protein Accession #: NP_001139318.1
• Nucleotide Accession #: NM_001145846.2