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Catalog No: OKCD02840
Price: $700.00
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Datasheets/ManualsPrintable datasheet for REG3A ELISA Kit (Mouse) (OKCD02840)
Product Info
Predicted Species ReactivityMouse|Mus musculus
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Serum (n=5)84-105%94-102%80-98%98-105%
EDTA Plasma (n=5)79-88%95-102%97-105%93-101%
Heparin Plasma (n=5)87-98%99-105%93-101%83-104%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Regenerating Islet Derived Protein 3 Alpha (REG3a). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Regenerating Islet Derived Protein 3 Alpha (REG3a). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Regenerating Islet Derived Protein 3 Alpha (REG3a), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Regenerating Islet Derived Protein 3 Alpha (REG3a) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range15.6-1000pg/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average(%)
Serum (n=5)90-10498
EDTA Plasma (n=5)80-10285
Heparin Plasma(n=5)96-105101
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Regenerating Islet Derived Protein 3 Alpha (REG3a) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Regenerating Islet Derived Protein 3 Alpha (REG3a) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
Anti-REG3A Microplate96 Wells (12 x 8 Well strips)
REG3A Lyophilized Standard2
100X Biotinylated REG3A Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
Stop Solution1 x 6 mL
TMB Substrate1 x 9 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma and other biological fluids
Sensitivity6.7 pg/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Regenerating Islet Derived Protein 3 Alpha (REG3a). No significant cross-reactivity or interference between Regenerating Islet Derived Protein 3 Alpha (REG3a) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Protocol Information
Gene SymbolReg3a
Gene Full Nameregenerating islet-derived 3 alpha
Alias SymbolsAV063448;generating islet-derived, mouse homolog 3 alpha;islet of Langerhans regenerating protein 3;lithostathine 3;pancreatitis-associated protein 2;reg III-alpha;REG-3-alpha;regenerating islet-derived protein 3-alpha;regenerating islet-derived protein III-alpha;RegIII (alpha).
NCBI Gene Id19694
Protein NameRegenerating islet-derived protein 3-alpha
Description of TargetBactericidal C-type lectin (By similarity). Regulates keratinocyte proliferation and differentiation after skin injury via activation of EXTL3-PI3K-AKT signaling pathway.
Uniprot IDO09037
Protein Accession #NP_035389.1
Nucleotide Accession #NM_011259.1
Protein Size (# AA)175
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