REG3A ELISA Kit (Human) (OKCD09577)

Catalog No: OKCD09577

Size:96WELLS

REG3A ELISA Kit (Human) (OKCD09577) Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Wide-range Regenerating Islet Derived Protein 3 Alpha (REG3a). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Wide-range Regenerating Islet Derived Protein 3 Alpha (REG3a). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Wide-range Regenerating Islet Derived Protein 3 Alpha (REG3a), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Wide-range Regenerating Islet Derived Protein 3 Alpha (REG3a) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 3.12-200ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Wide-range Regenerating Islet Derived Protein 3 Alpha (REG3a) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Wide-range Regenerating Islet Derived Protein 3 Alpha (REG3a) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • REG3A Microplate: 96 Wells (12 x 8 Well strips)
  • REG3A Lyophilized Standard: 2
  • 100X REG3A HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Sensitivity: < 1.14ng/mL

Target Info

• Gene Symbol: REG3A
• Gene Full Name: regenerating family member 3 alpha
• Alias Symbols: hepatocarcinoma-intestine-pancreas;hepatointestinal pancreatic protein;HIP;HIP/PAP;human proislet peptide;INGAP;pancreatic beta cell growth factor;pancreatitis-associated protein 1;PAP;PAP homologous protein;PAP1;PAP-H;PBCGF;proliferation-inducing protein 34;proliferation-inducing protein 42;reg III-alpha;REG3;REG-3-alpha;regenerating islet-derived 3 alpha;regenerating islet-derived protein 3-alpha;regenerating islet-derived protein III-alpha;REG-III.
• NCBI Gene Id: 5068
• Protein Name: Regenerating islet-derived protein 3-alpha
• Description of Target: Bactericidal C-type lectin which acts exclusively against Gram-positive bacteria and mediates bacterial killing by binding to surface-exposed carbohydrate moieties of peptidoglycan. Regulates keratinocyte proliferation and differentiation after skin injury via activation of EXTL3-PI3K-AKT signaling pathway.
• Uniprot ID: Q06141
• Protein Accession #: NP_002571.1
• Nucleotide Accession #: NM_002580.2