REG1B ELISA Kit (Human) (OKCD08682)

Catalog No: OKCD08682

Size:96WELLS

REG1B ELISA Kit (Human) (OKCD08682) Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Regenerating Islet Derived Protein 1 Beta (REG1b). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Regenerating Islet Derived Protein 1 Beta (REG1b). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Regenerating Islet Derived Protein 1 Beta (REG1b), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Regenerating Islet Derived Protein 1 Beta (REG1b) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 1.56-100ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Regenerating Islet Derived Protein 1 Beta (REG1b) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Regenerating Islet Derived Protein 1 Beta (REG1b) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • REG1B Microplate: 96 Wells (12 x 8 Well strips)
  • REG1B Lyophilized Standard: 2
  • 100X REG1B HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 2 mL
  • Stop Solution: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Sensitivity: < 0.65ng/mL

Target Info

• Gene Symbol: REG1B
• Gene Full Name: regenerating family member 1 beta
• Alias Symbols: lithostathine-1-beta;pancreatic stone protein 2;PSP-2;PSPS2;REG-1-beta;regenerating islet-derived 1 beta (pancreatic stone protein, pancreatic thread protein);regenerating islet-derived protein 1-beta;regenerating protein I beta;REGH;REGI-BETA;REGL;secretory pancreatic stone protein 2.
• NCBI Gene Id: 5968
• Protein Name: Lithostathine-1-beta
• Description of Target: Might act as an inhibitor of spontaneous calcium carbonate precipitation. May be associated with neuronal sprouting in brain, and with brain and pancreas regeneration.
• Uniprot ID: P48304
• Protein Accession #: NP_006498.1
• Nucleotide Accession #: NM_006507.3